دورية أكاديمية
أعرض في EDS

Evidence of colistin resistance genes ( mcr-1 and mcr-2 ) in wild birds and its public health implication in Egypt.

التفاصيل البيبلوغرافية
العنوان: Evidence of colistin resistance genes ( mcr-1 and mcr-2 ) in wild birds and its public health implication in Egypt.
المؤلفون: Ahmed ZS; 1Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt., Elshafiee EA; 1Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt., Khalefa HS; 2Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt., Kadry M; 1Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt., Hamza DA; 1Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt.
المصدر: Antimicrobial resistance and infection control [Antimicrob Resist Infect Control] 2019 Dec 03; Vol. 8, pp. 197. Date of Electronic Publication: 2019 Dec 03 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101585411 Publication Model: eCollection Cited Medium: Internet ISSN: 2047-2994 (Electronic) Linking ISSN: 20472994 NLM ISO Abbreviation: Antimicrob Resist Infect Control Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Bacteria/*drug effects , Bacterial Proteins/*genetics , Birds/*microbiology , Colistin/*pharmacology , Drug Resistance, Bacterial/*genetics, Animals ; Animals, Wild/microbiology ; Bacteria/classification ; Bacteria/genetics ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/genetics ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Feces/microbiology ; Humans ; Klebsiella/genetics ; Microbial Sensitivity Tests ; Plasmids/genetics ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; Public Health ; Water Microbiology
مستخلص: Background: Antimicrobial resistance has become one of the most severe global threats to human and veterinary Medicin e. coli stin is an effective therapeutic agent against multi-drug-resistant pathogens. However, the discovery of transferable plasmids that confer resistance to colistin ( mcr-1) has led to challenges in medical science. This study describes the role of wild birds in the harbouring and environmental spread of colistin-resistant bacteria, which could pose a potential hazard to human and animal health.
Methods: In total, 140 faecal samples from wild birds (migratory and resident birds) were tested. Twenty surface water samples were collected from the area in which wild bird trapping was conducted, and 50 human stool samples were collected from individuals residing near the surface water sources and farm buildings. Isolation and identification of Enterobacteriaceae and Pseudomonas aeruginosa from the different samples were performed using conventional culture techniques and biochemical identification. PCR amplification of the mcr genes was performed in all positive isolates. Sequencing of mcr -1 genes from three randomly selected E. coli carrying mcr-1 isolates; wild birds, water and humans was performed.
Result: The bacteriological examination of the samples showing isolates of Escherichia coli , Klebsiella pneumoniae , Klebsiella oxytoca and P. aeruginosa . The results of multiplex PCR of the mcr genes revealed that E. coli was the most prevalent gram-negative bacterium harbouring the mcr genes, whereas a low prevalence was observed for K. pneumoniae . The prevalence of mcr- 1 in resident birds, migratory birds, water sources and humans were 10.4, 20,16.6 and 9.6% while the prevalence of mcr -2 were 1.4, 3.6, 11.1 and 9.6%, respectively. Sequencing of the mcr-1 gene from the three E. coli carrying mcr - 1 isolates indicated a possible correlation between the wild bird and surface water isolates.
Conclusion: The detection of mcr -1-positive bacteria in wild birds in Egypt indicates the possible environmental dissemination of this gene through bird activity. The impact of the interaction between domestic and wild animals on public health cannot be overlooked.
Competing Interests: Competing interestsThe authors declare that they have no competing interests.
(© The Author(s). 2019.)
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فهرسة مساهمة: Keywords: Egypt; Enterobacteriaceae; P. aeruginosa; Wild bird; mcr genes
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Escherichia coli Proteins)
0 (MCR-1 protein, E coli)
Z67X93HJG1 (Colistin)
تواريخ الأحداث: Date Created: 20191213 Date Completed: 20200717 Latest Revision: 20240722
رمز التحديث: 20240722
مُعرف محوري في PubMed: PMC6892208
DOI: 10.1186/s13756-019-0657-5
PMID: 31827778
قاعدة البيانات: MEDLINE
الوصف
تدمد:2047-2994
DOI:10.1186/s13756-019-0657-5