دورية أكاديمية
Structural Basis for Finding OG Lesions and Avoiding Undamaged G by the DNA Glycosylase MutY.
العنوان: | Structural Basis for Finding OG Lesions and Avoiding Undamaged G by the DNA Glycosylase MutY. |
---|---|
المؤلفون: | Russelburg LP; School of Biological Sciences , University of Utah , 257 South 1400 East , Salt Lake City , Utah 84112 , United States., O'Shea Murray VL; Department of Chemistry , University of California , Davis , California 95616 , United States.; Department of Chemistry , University of Utah , 315 South 1400 East , Salt Lake City , Utah 84112 , United States., Demir M; Department of Chemistry , University of California , Davis , California 95616 , United States., Knutsen KR; School of Biological Sciences , University of Utah , 257 South 1400 East , Salt Lake City , Utah 84112 , United States., Sehgal SL; School of Biological Sciences , University of Utah , 257 South 1400 East , Salt Lake City , Utah 84112 , United States., Cao S; Department of Chemistry , University of California , Davis , California 95616 , United States., David SS; Department of Chemistry , University of California , Davis , California 95616 , United States., Horvath MP; School of Biological Sciences , University of Utah , 257 South 1400 East , Salt Lake City , Utah 84112 , United States. |
المصدر: | ACS chemical biology [ACS Chem Biol] 2020 Jan 17; Vol. 15 (1), pp. 93-102. Date of Electronic Publication: 2019 Dec 27. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8937 (Electronic) Linking ISSN: 15548929 NLM ISO Abbreviation: ACS Chem Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2006- |
مواضيع طبية MeSH: | DNA/*chemistry , DNA Glycosylases/*metabolism , DNA Repair/*drug effects , Targeted Gene Repair/*methods, Amino Acid Sequence ; Base Pair Mismatch ; Catalytic Domain ; Guanine/chemistry ; Kinetics ; Molecular Conformation ; Structure-Activity Relationship ; Substrate Specificity |
مستخلص: | The adenine glycosylase MutY selectively initiates repair of OG:A lesions and, by comparison, avoids G:A mispairs. The ability to distinguish these closely related substrates relies on the C-terminal domain of MutY, which structurally resembles MutT. To understand the mechanism for substrate specificity, we crystallized MutY in complex with DNA containing G across from the high-affinity azaribose transition state analogue. Our structure shows that G is accommodated by the OG site and highlights the role of a serine residue in OG versus G discrimination. The functional significance of Ser308 and its neighboring residues was evaluated by mutational analysis, revealing the critical importance of a β loop in the C-terminal domain for mutation suppression in cells, and biochemical performance in vitro . This loop comprising residues Phe307, Ser308, and His309 ( Geobacillus stearothermophilus sequence positions) is conserved in MutY but absent in MutT and other DNA repair enzymes and may therefore serve as a MutY-specific target exploitable by chemical biological probes. |
References: | J Bacteriol. 1992 Oct;174(20):6321-5. (PMID: 1328155) ACS Chem Biol. 2013 Jan 18;8(1):71-81. (PMID: 23259601) DNA Repair (Amst). 2018 Nov;71:118-126. (PMID: 30228084) Bioorg Med Chem Lett. 2011 Sep 1;21(17):4969-72. (PMID: 21689934) Biochemistry. 2005 May 3;44(17):6670-4. (PMID: 15850400) Proc Natl Acad Sci U S A. 1989 Nov;86(22):8877-81. (PMID: 2682664) Biochemistry. 2000 Jun 27;39(25):7331-6. (PMID: 10858279) Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002) Nat Genet. 2002 Feb;30(2):227-32. (PMID: 11818965) Nature. 2005 Mar 31;434(7033):612-8. (PMID: 15800616) Biochemistry. 2004 Jan 27;43(3):651-62. (PMID: 14730969) Biochemistry. 2003 Jan 28;42(3):801-10. (PMID: 12534293) Biochemistry. 1998 Oct 20;37(42):14756-64. (PMID: 9778350) Nucleic Acids Res. 2001 Jan 15;29(2):553-64. (PMID: 11139626) Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18497-502. (PMID: 19841264) Free Radic Biol Med. 2017 Jun;107:202-215. (PMID: 28087410) Arch Biochem Biophys. 2000 Aug 1;380(1):11-9. (PMID: 10900127) J Biol Chem. 2000 Mar 24;275(12):8448-55. (PMID: 10722679) ACS Chem Biol. 2015 Oct 16;10(10):2334-43. (PMID: 26218629) Nature. 2004 Feb 12;427(6975):652-6. (PMID: 14961129) J Appl Crystallogr. 2013 Feb 1;46(Pt 1):1-13. (PMID: 23396808) J Biol Chem. 2017 Mar 24;292(12):5007-5017. (PMID: 28130451) Nat Rev Cancer. 2012 Dec;12(12):801-17. (PMID: 23175119) J Biol Chem. 2015 Jul 10;290(28):17096-105. (PMID: 25995449) DNA Repair (Amst). 2019 Aug;80:16-25. (PMID: 31203172) ACS Chem Biol. 2017 Sep 15;12(9):2335-2344. (PMID: 28723094) J Bacteriol. 2007 Feb;189(3):902-10. (PMID: 17114250) Biochemistry. 1999 May 18;38(20):6374-9. (PMID: 10350454) Oncotarget. 2017 Feb 7;8(6):9216-9229. (PMID: 27999205) Nat Chem Biol. 2008 Jan;4(1):51-8. (PMID: 18026095) J Am Chem Soc. 2018 Feb 14;140(6):2105-2114. (PMID: 29376367) ACS Omega. 2019 Jul 05;4(7):11642-11656. (PMID: 31460271) Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. (PMID: 20124702) Biochemistry. 1996 Dec 24;35(51):16665-71. (PMID: 8988002) Nucleic Acids Res. 2016 Jan 29;44(2):801-10. (PMID: 26673696) Nature. 1992 Jan 16;355(6357):273-5. (PMID: 1309939) J Bacteriol. 2000 Jun;182(12):3353-60. (PMID: 10852864) Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7022-5. (PMID: 1495996) Methods Mol Biol. 2014;1079:263-71. (PMID: 24170408) Biochemistry. 1992 Nov 17;31(45):10964-8. (PMID: 1445834) ACS Chem Biol. 2019 Jan 18;14(1):27-36. (PMID: 30500207) Nucleic Acids Res. 2018 Jan 4;46(D1):D1062-D1067. (PMID: 29165669) Nucleic Acids Res. 2008 Apr;36(7):2295-300. (PMID: 18287115) Nature. 1993 Apr 22;362(6422):709-15. (PMID: 8469282) |
معلومات مُعتمدة: | T32 GM008537 United States GM NIGMS NIH HHS; R01 CA067985 United States CA NCI NIH HHS; P30 GM124169 United States GM NIGMS NIH HHS; S10 OD018483 United States OD NIH HHS; T32 CA093247 United States CA NCI NIH HHS; R29 CA067985 United States CA NCI NIH HHS |
المشرفين على المادة: | 5Z93L87A1R (Guanine) 9007-49-2 (DNA) EC 3.2.2.- (DNA Glycosylases) EC 3.2.2.- (mutY adenine glycosylase) |
تواريخ الأحداث: | Date Created: 20191213 Date Completed: 20210113 Latest Revision: 20210118 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC7069122 |
DOI: | 10.1021/acschembio.9b00639 |
PMID: | 31829624 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1554-8937 |
---|---|
DOI: | 10.1021/acschembio.9b00639 |