دورية أكاديمية
أعرض في EDS

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata.

التفاصيل البيبلوغرافية
العنوان: Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata.
المؤلفون: Silva SFMD; Department of Morphology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Silva CHS; Department of Morphology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Dias FCR; Department of General Biology, Federal University of Viçosa-UFV, Viçosa, Minas Gerais, Brazil., Cordero-Schmidt E; Department of Ecology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Vargas-Mena JC; Department of Ecology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Silva IGMD; Department of Cell Biology, University of Brasília-UnB, Brasília, Distrito Federal, Brazil., Báo SN; Department of Cell Biology, University of Brasília-UnB, Brasília, Distrito Federal, Brazil., Carvalho TG; Department of Morphology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Araújo Júnior RF; Department of Morphology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil., Moura CEB; Department of Animal Sciences, Federal Rural University of the Semi-Arid Region-UFERSA, Mossoró, Rio Grande do Norte, Brazil., Melo FCSA; Department of Animal Biology, Federal University of Viçosa-UFV, Viçosa, Minas Gerais, Brazil., Matta SLPD; Department of General Biology, Federal University of Viçosa-UFV, Viçosa, Minas Gerais, Brazil., Morais DB; Department of Morphology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande do Norte, Brazil.
المصدر: PloS one [PLoS One] 2019 Dec 13; Vol. 14 (12), pp. e0226558. Date of Electronic Publication: 2019 Dec 13 (Print Publication: 2019).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Spermatogenesis*, Biomarkers/*metabolism , Chiroptera/*physiology , Testis/*physiology, Animals ; Cell Nucleus/metabolism ; Leydig Cells/cytology ; Leydig Cells/metabolism ; Male ; Seminiferous Tubules/cytology ; Seminiferous Tubules/metabolism ; Sertoli Cells/cytology ; Sertoli Cells/metabolism ; Testis/anatomy & histology
مستخلص: Diphylla ecaudata is a hematophagous bat endemic of South America, with food preference for bird blood. Given the lack of information about the reproductive activity of this species, this study aimed to describe the testicular morphology and histomorphometry of D. ecaudata in order to understand its reproductive biology, specially spermatogenesis. The animals were collected in Lajes city, Rio Grande do Norte, Brazil. Following euthanasia, the testes were histologically processed for morphological, morphometric, ultrastructural and immunohistochemical analyses. Their average body weight was 24.64g, with a gonadosomatic index of 0.49%, tubulesomatic index of 0.47%, and a total of 32.20m of seminiferous tubules per gram of testis. The pre-meiotic, meiotic, and post-meiotic phases accounted for 56.20%, 9.30%, and 34.50% of the seminiferous epithelium cycle, respectively. The ultrastructure of spermiogenesis was similar to that described in other mammals and the perforatorium was not observed in the sperm. Androgen receptors were detected in Sertoli cell nuclei and Leydig cell cytoplasm, while aromatase enzyme was detected only in Sertoli cell nuclei. FGF2 and BCL-2 activities were detected in the cytoplasm of zygotene and pachytene primary spermatocytes, as well as round and elongated spermatids. D. ecaudata showed testicular pattern similar to other mammals and characteristics common to other bat species. This species stood out for its high efficiency of Sertoli cells, which presented high capacity to support germ cells, besides the highest sperm production rates among those already recorded. This study is the first step towards the knowledge of D. ecaudata reproduction and the first description of its spermatogenesis.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Biomarkers)
تواريخ الأحداث: Date Created: 20191214 Date Completed: 20200402 Latest Revision: 20200402
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6910855
DOI: 10.1371/journal.pone.0226558
PMID: 31835274
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0226558