دورية أكاديمية
أعرض في EDS

Identification of miR-328-3p as an endogenous reference gene for the normalization of miRNA expression data from patients with Diabetic Retinopathy.

التفاصيل البيبلوغرافية
العنوان: Identification of miR-328-3p as an endogenous reference gene for the normalization of miRNA expression data from patients with Diabetic Retinopathy.
المؤلفون: Prado MSG; Department of Health Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil., de Goes TC; Department of Biological Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil., de Jesus ML; Department of Health Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil., Mendonça LSO; Department of Biological Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil., Nascimento JS; Department of Health Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil., Kaneto CM; Department of Biological Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil. cmkaneto@uesc.br.
المصدر: Scientific reports [Sci Rep] 2019 Dec 23; Vol. 9 (1), pp. 19677. Date of Electronic Publication: 2019 Dec 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Diabetic Retinopathy/*genetics , MicroRNAs/*genetics, Adult ; Aged ; Biomarkers/blood ; Case-Control Studies ; Circulating MicroRNA/blood ; Circulating MicroRNA/genetics ; Diabetes Mellitus/blood ; Diabetes Mellitus/genetics ; Diabetic Retinopathy/blood ; Female ; Gene Expression Profiling ; Genetic Markers ; Humans ; Male ; MicroRNAs/blood ; Middle Aged ; RNA Stability ; Real-Time Polymerase Chain Reaction
مستخلص: Diabetic Retinopathy, the main cause of visual loss and blindness among working population, is a complication of Diabetes mellitus (DM), which has been described as a major public health challenge, so it is important to identify biomarkers to predict and to stratify patient´s possibility for developing DR. MicroRNAs (miRNAs) are small non-coding RNA molecules that have showed to be promising disease biomarkers and association of miRNAs with the possibility to develop DR has been reported. However, evaluating miRNA expression involves normalization of RT-qPCR data using internal reference genes that should be properly determined, considering their impact on expression levels calculation and, until date, there is no unanimity on reference miRNAs for the investigation of circulating miRNAs in DR. We aimed to estimate the appropriateness of a group of miRNAs as normalizers to identify which might be considered steady internal reference genes in expression studies on DR plasma samples. Expression levels of candidates were analyzed in 60 healthy controls, 48 DM without DR patients and 62 DR patients with two statistical tools: NormFinder and RefFinder. MiR-328-3p was the most stable gene and we also investigated the effect of gene normalization, demonstrating that different normalization strategies have important implications for accurate data interpretation.
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المشرفين على المادة: 0 (Biomarkers)
0 (Circulating MicroRNA)
0 (Genetic Markers)
0 (MIRN328 microRNA, human)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20191225 Date Completed: 20201109 Latest Revision: 20210110
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6928074
DOI: 10.1038/s41598-019-56172-w
PMID: 31873160
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-019-56172-w