دورية أكاديمية
Fish oil up-regulates hepatic autophagy in rats with chronic ethanol consumption.
| العنوان: | Fish oil up-regulates hepatic autophagy in rats with chronic ethanol consumption. |
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| المؤلفون: | Lu NS; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan., Chiu WC; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan., Chen YL; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan., Peng HC; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan., Shirakawa H; Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan., Yang SC; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan; Research Center of Geriatric Nutrition, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan; Nutrition Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan. Electronic address: sokei@tmu.edu.tw. |
| المصدر: | The Journal of nutritional biochemistry [J Nutr Biochem] 2020 Mar; Vol. 77, pp. 108314. Date of Electronic Publication: 2019 Dec 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9010081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4847 (Electronic) Linking ISSN: 09552863 NLM ISO Abbreviation: J Nutr Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1996->: New York, NY : Elsevier Science Original Publication: Stoneham, MA, USA : Butterworths, c1990- |
| مواضيع طبية MeSH: | Autophagy* , Oxidative Stress*, Alcohol Drinking/*adverse effects , Fish Oils/*pharmacology , Liver/*metabolism, Alanine Transaminase/metabolism ; Animals ; Antioxidants/metabolism ; Aspartate Aminotransferases/metabolism ; Autophagy-Related Protein-1 Homolog/metabolism ; Beclin-1/metabolism ; Cholesterol/metabolism ; Cytochrome P-450 CYP2E1/metabolism ; Dietary Supplements ; Disease Models, Animal ; Ethanol ; Glutathione/metabolism ; Inflammation ; Lipid Metabolism ; Liver Diseases, Alcoholic/metabolism ; Male ; Olive Oil/pharmacology ; Rats ; Rats, Wistar ; Triglycerides/metabolism ; Up-Regulation |
| مستخلص: | In this study, we examined the regulation of autophagy by fish oil in rats under ethanol-containing diets. Thirty male Wistar rats (8-week-old) were divided into six groups and fed a control diet or an ethanol-containing diet, which was adjusted with fish oil to replace 25% or 57% of the olive oil. After 8 weeks, rats in the E (ethanol diet) group showed the significantly higher plasma aspartate transaminase (AST) and alanine transaminase (ALT) activities, protein expression of cytochrome P450 2E1 (CYP2E1), and levels of hepatic inflammatory cytokines. However, all of those items had significantly decreased in the EF25 (ethanol with 25% fish oil) and EF57 (ethanol with 57% fish oil) groups. As to autophagic indicators, protein expressions of mammalian target of rapamycin (mTOR), Unc-51-like autophagy activating kinase 1 (ULK1) and p62 were significantly increased in the E group. Conversely, the protein expressions of light chain 3II (LC3II)/LC3I and Beclin1 were significantly decreased in the E group. On the other hand, protein expressions of phosphorylated Akt, mTOR, ULK1, and p62 were down-regulated, protein expressions of LC3II/LC3I and Beclin1 were conversely up-regulated in the EF25 and EF57 groups. Fish oil activated hepatic autophagy via inhibiting the Akt signaling pathway, which exerted protective effects against ethanol-induced liver injury in rats. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Akt singalling pathway; Alcoholic liver disease; Autophagy; Fish oil; Wistar rats |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Beclin-1) 0 (Becn1 protein, rat) 0 (Fish Oils) 0 (Olive Oil) 0 (Triglycerides) 3K9958V90M (Ethanol) 97C5T2UQ7J (Cholesterol) EC 1.14.13.- (Cytochrome P-450 CYP2E1) EC 2.6.1.1 (Aspartate Aminotransferases) EC 2.6.1.2 (Alanine Transaminase) EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog) EC 2.7.11.1 (ULK1 protein, rat) GAN16C9B8O (Glutathione) |
| تواريخ الأحداث: | Date Created: 20191230 Date Completed: 20210224 Latest Revision: 20210224 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jnutbio.2019.108314 |
| PMID: | 31884243 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4847 |
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| DOI: | 10.1016/j.jnutbio.2019.108314 |