دورية أكاديمية
أعرض في EDS

Synergistic anticandidal activity of etomidate and azoles against clinical fluconazole-resistant Candida isolates.

التفاصيل البيبلوغرافية
العنوان: Synergistic anticandidal activity of etomidate and azoles against clinical fluconazole-resistant Candida isolates.
المؤلفون: do Av Sá LG; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., da Silva CR; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., S Campos R; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil.; University Center Christus, Fortaleza, CE 60160-230, Brazil., de A Neto JB; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil.; University Center Christus, Fortaleza, CE 60160-230, Brazil., Sampaio LS; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., do Nascimento FB; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Barroso FD; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., da Silva LJ; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Queiroz HA; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Cândido TM; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil.; University Center Christus, Fortaleza, CE 60160-230, Brazil., Rodrigues DS; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Leitão AC; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., de Moraes MO; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Cavalcanti BC; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil., Júnior HV; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE 60430-1160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE 60430-276, Brazil.
المصدر: Future microbiology [Future Microbiol] 2019 Nov; Vol. 14, pp. 1477-1488. Date of Electronic Publication: 2020 Jan 09.
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Future Medicine Country of Publication: England NLM ID: 101278120 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1746-0921 (Electronic) Linking ISSN: 17460913 NLM ISO Abbreviation: Future Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Future Medicine
مواضيع طبية MeSH: Drug Resistance, Fungal*, Antifungal Agents/*pharmacology , Azoles/*pharmacology , Candida/*drug effects , Etomidate/*pharmacology , Fluconazole/*pharmacology, Animals ; Biofilms/drug effects ; Cell Survival/drug effects ; Cricetinae ; DNA Damage/drug effects ; Drug Discovery ; Drug Synergism ; Fibroblasts/drug effects ; Lung/cytology ; Lung/drug effects ; Membrane Potential, Mitochondrial/drug effects ; Microbial Sensitivity Tests
مستخلص: Aim: The purpose of this study was to evaluate the effect of etomidate alone and in combination with azoles on resistant strains of Candida spp. in both planktonic cells and biofilms. Materials & methods: The antifungal activity of etomidate was assessed by the broth microdilution test; flow cytometric procedures to measure fungal viability, mitochondrial transmembrane potential, free radical generation and cell death; as well detection of DNA damage using the comet assay. The interaction between etomidate and antifungal drugs (itraconazole and fluconazole) was evaluated by the checkerboard assay. Results: Etomidate showed antifungal activity against resistant strains of Candida spp. in planktonic cells and biofilms. Etomidate also presented synergism with fluconazole and itraconazole in planktonic cells and biofilms. Conclusion: Etomidate showed antifungal activity against Candida spp., indicating that it is a possible therapeutic alternative.
فهرسة مساهمة: Keywords: Candida spp.; azoles; biofilm; etomidate; synergism
المشرفين على المادة: 0 (Antifungal Agents)
0 (Azoles)
8VZV102JFY (Fluconazole)
Z22628B598 (Etomidate)
تواريخ الأحداث: Date Created: 20200110 Date Completed: 20200528 Latest Revision: 20200528
رمز التحديث: 20240628
DOI: 10.2217/fmb-2019-0075
PMID: 31916846
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-0921
DOI:10.2217/fmb-2019-0075