دورية أكاديمية
Detection and in vitro studies of Cucurbita maxima phloem serpin-1 RNA-binding properties.
| العنوان: | Detection and in vitro studies of Cucurbita maxima phloem serpin-1 RNA-binding properties. |
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| المؤلفون: | Tolstyko EA; Department of Virology, Biological Faculty, Moscow State University, Moscow, 119234, Russia., Lezzhov AA; Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119991, Russia., Pankratenko AV; Department of Virology, Biological Faculty, Moscow State University, Moscow, 119234, Russia., Serebryakova MV; Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia., Solovyev AG; Department of Virology, Biological Faculty, Moscow State University, Moscow, 119234, Russia; Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, 119991, Russia. Electronic address: solovyev@belozersky.msu.ru., Morozov SY; Department of Virology, Biological Faculty, Moscow State University, Moscow, 119234, Russia; Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia. |
| المصدر: | Biochimie [Biochimie] 2020 Mar; Vol. 170, pp. 118-127. Date of Electronic Publication: 2020 Jan 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 1264604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1638-6183 (Electronic) Linking ISSN: 03009084 NLM ISO Abbreviation: Biochimie Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Paris : Editions Scientifiques Elsevier Original Publication: Paris. |
| مواضيع طبية MeSH: | MicroRNAs/*metabolism , Plant Proteins/*metabolism , RNA, Messenger/*metabolism , RNA, Plant/*metabolism , RNA, Transfer/*metabolism , RNA-Binding Proteins/*metabolism , Serpins/*metabolism, Amino Acid Sequence ; Cucurbita/metabolism ; In Vitro Techniques ; MicroRNAs/genetics ; Plant Proteins/genetics ; RNA, Messenger/genetics ; RNA, Plant/genetics ; RNA, Transfer/genetics ; RNA-Binding Proteins/genetics ; Sequence Homology ; Serpins/genetics |
| مستخلص: | Apart from being a conduit for photoassimilate transport in plants, the phloem serves as a pathway for transport of proteins and RNAs from sites of their synthesis to distant plant parts. As demonstrated for mRNAs and small RNAs such as miRNA and siRNA, their phloem transport is largely involved in responses to environmental cues including stresses and pathogen attacks. RNA molecules are believed to be transported in the phloem in the form of complexes with RNA-binding proteins; however, proteins forming such complexes are generally poorly studied. Here, we demonstrate that the Cucurbita maxima phloem serpin-1 (CmPS1), which has been previously described as a functional protease inhibitor capable of long-distance transport via the phloem, is able to bind RNA in vitro. Among different RNAs tested, CmPS1 exhibits a preference for imperfect RNA duplexes and the highest affinity to tRNA. A characteristic complex formed by CmPS1 with tRNA is not observed upon CmPS1 binding to tRNA-like structures of plant viruses. Mutational analysis demonstrates that the CmPS1 N-terminal region is not involved in RNA binding. Since antithrombin-III, the human protease inhibitor of serpin family most closely sequence-related to CmPS1, is found to be unable to bind RNA, one can suggest that, in its evolution, CmPS1 has gained the RNA binding capability as an additional function likely relevant to its specific activities in the plant phloem. Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest. (Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.) |
| فهرسة مساهمة: | Keywords: Phloem; Protease inhibitor; RNA-Binding protein; Serpin; tRNA |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Plant Proteins) 0 (RNA, Messenger) 0 (RNA, Plant) 0 (RNA-Binding Proteins) 0 (Serpins) 0 (phloem serpin 1) 9014-25-9 (RNA, Transfer) |
| تواريخ الأحداث: | Date Created: 20200115 Date Completed: 20201117 Latest Revision: 20201117 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.biochi.2020.01.006 |
| PMID: | 31935442 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1638-6183 |
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| DOI: | 10.1016/j.biochi.2020.01.006 |