دورية أكاديمية
Nanoparticle Beacons: Supersensitive Smart Materials with On/Off-Switchable Affinity to Biomedical Targets.
| العنوان: | Nanoparticle Beacons: Supersensitive Smart Materials with On/Off-Switchable Affinity to Biomedical Targets. |
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| المؤلفون: | Cherkasov VR; Moscow Institute of Physics and Technology , 9 Institutskii per. , 141700 Dolgoprudny, Moscow Region , Russia.; Prokhorov General Physics Institute of the Russian Academy of Sciences , 38 Vavilov St , 119991 Moscow , Russia., Mochalova EN; Moscow Institute of Physics and Technology , 9 Institutskii per. , 141700 Dolgoprudny, Moscow Region , Russia.; Prokhorov General Physics Institute of the Russian Academy of Sciences , 38 Vavilov St , 119991 Moscow , Russia., Babenyshev AV; Moscow Institute of Physics and Technology , 9 Institutskii per. , 141700 Dolgoprudny, Moscow Region , Russia.; Prokhorov General Physics Institute of the Russian Academy of Sciences , 38 Vavilov St , 119991 Moscow , Russia., Vasilyeva AV; Moscow Institute of Physics and Technology , 9 Institutskii per. , 141700 Dolgoprudny, Moscow Region , Russia., Nikitin PI; Prokhorov General Physics Institute of the Russian Academy of Sciences , 38 Vavilov St , 119991 Moscow , Russia., Nikitin MP; Moscow Institute of Physics and Technology , 9 Institutskii per. , 141700 Dolgoprudny, Moscow Region , Russia. |
| المصدر: | ACS nano [ACS Nano] 2020 Feb 25; Vol. 14 (2), pp. 1792-1803. Date of Electronic Publication: 2020 Jan 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington D.C. : American Chemical Society |
| مواضيع طبية MeSH: | DNA/*analysis , Gold/*chemistry , Metal Nanoparticles/*chemistry , Smart Materials/*chemistry, Drug Delivery Systems ; Particle Size ; Polymers/chemistry ; Surface Properties |
| مستخلص: | Smart materials that can switch between different states under the influence of chemical triggers are highly demanded in biomedicine, where specific responsiveness to biomarkers is imperative for precise diagnostics and therapy. Superior selectivity of drug delivery to malignant cells may be achieved with the nanoagents that stay "inert" until "activation" by the characteristic profile of microenvironment cues ( e.g., tumor metabolites, angiogenesis factors, microRNA/DNA, etc. ). However, despite a wide variety and functional complexity of smart material designs, their real-life applications are hindered by very limited sensitivity to inputs. Here, we present ultrasensitive smart nanoagents with input-dependent On/Off switchable affinity to a biomedical target based on a combination of gold nanoparticles with low-energy polymer structures. In the proposed method, a nanoparticle-based agent is surface coated with a custom designed flexible polymer chain, which has an input-switchable structure that regulates accessibility of the terminal receptor for target binding. Implementation of the concept with a DNA-model of such polymer has yielded nanoagents that have input-dependent cell-targeting capabilities and responsiveness to as little as 30 fM of DNA input in 15 min lateral flow assay. Thus, we show that surface phenomena can augment nanoagents with capability for switchable affinity without compromising the sensitivity to inputs. The proposed approach is promising for development of next-generation theranostic agents and ultrasensitive nanosensors for point-of-care diagnostics. |
| فهرسة مساهمة: | Keywords: DNA assay; cell targeting; gold nanoparticles; lateral flow assay; molecular beacons; smart materials; theranostic agents |
| المشرفين على المادة: | 0 (Polymers) 0 (Smart Materials) 7440-57-5 (Gold) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20200117 Date Completed: 20201218 Latest Revision: 20201218 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acsnano.9b07569 |
| PMID: | 31944662 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1936-086X |
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| DOI: | 10.1021/acsnano.9b07569 |