دورية أكاديمية
Composition-function analysis of HDL subpopulations: influence of lipid composition on particle functionality.
| العنوان: | Composition-function analysis of HDL subpopulations: influence of lipid composition on particle functionality. |
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| المؤلفون: | Niisuke K; Cardiovascular Nutrition Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111., Kuklenyik Z; Centers for Disease Control and Prevention, Division of Laboratory Sciences, Atlanta, GA 30341., Horvath KV; Cardiovascular Nutrition Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111., Gardner MS; Centers for Disease Control and Prevention, Division of Laboratory Sciences, Atlanta, GA 30341., Toth CA; Centers for Disease Control and Prevention, Division of Laboratory Sciences, Atlanta, GA 30341., Asztalos BF; Cardiovascular Nutrition Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111. |
| المصدر: | Journal of lipid research [J Lipid Res] 2020 Mar; Vol. 61 (3), pp. 306-315. Date of Electronic Publication: 2020 Jan 17. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0376606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-7262 (Electronic) Linking ISSN: 00222275 NLM ISO Abbreviation: J Lipid Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: Memphis, Lipid Research, inc. |
| مواضيع طبية MeSH: | Coronary Disease/*blood , Lipid Droplets/*chemistry , Lipoproteins, HDL/*blood, Adult ; Aged ; Coronary Disease/metabolism ; Female ; Humans ; Lipid Droplets/metabolism ; Lipoproteins, HDL/metabolism ; Male ; Middle Aged ; Particle Size ; Young Adult |
| مستخلص: | The composition-function relationship of HDL particles and its effects on the mechanisms driving coronary heart disease (CHD) is poorly understood. We tested the hypothesis that the functionality of HDL particles is significantly influenced by their lipid composition. Using a novel 3D-separation method, we isolated five different-sized HDL subpopulations from CHD patients who had low preβ-1 functionality (low-F) (ABCA1-dependent cholesterol-efflux normalized for preβ-1 concentration) and controls who had either low-F or high preβ-1 functionality (high-F). Molecular numbers of apoA-I, apoA-II, and eight major lipid classes were determined in each subpopulation by LC-MS. The average number of lipid molecules decreased from 422 in the large spherical α-1 particles to 57 in the small discoid preβ-1 particles. With decreasing particle size, the relative concentration of free cholesterol (FC) decreased in α-mobility but not in preβ-1 particles. Preβ-1 particles contained more lipids than predicted; 30% of which were neutral lipids (cholesteryl ester and triglyceride), indicating that these particles were mainly remodeled from larger particles not newly synthesized. There were significant correlations between HDL-particle functionality and the concentrations of several lipids. Unexpectedly, the phospholipid:FC ratio was significantly correlated with large-HDL-particle functionality but not with preβ-1 functionality. There was significant positive correlation between particle functionality and total lipids in high-F controls, indicating that the lipid-binding capacity of apoA-I plays a major role in the cholesterol efflux capacity of HDL particles. Functionality and lipid composition of HDL particles are significantly correlated and probably both are influenced by the lipid-binding capacity of apoA-I. |
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| معلومات مُعتمدة: | R01 HL117933 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: high density lipoprotein; lipid ratios; phospholipids; pre-beta |
| المشرفين على المادة: | 0 (Lipoproteins, HDL) |
| تواريخ الأحداث: | Date Created: 20200119 Date Completed: 20210603 Latest Revision: 20240328 |
| رمز التحديث: | 20240329 |
| مُعرف محوري في PubMed: | PMC7053829 |
| DOI: | 10.1194/jlr.RA119000258 |
| PMID: | 31953305 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1539-7262 |
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| DOI: | 10.1194/jlr.RA119000258 |