دورية أكاديمية
Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells.
| العنوان: | Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells. |
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| المؤلفون: | van Essen MF; Div. of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands. Electronic address: m.f.van_essen@lumc.nl., Schlagwein N; Div. of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands., van Gijlswijk-Janssen DJ; Div. of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands., Anholts JDH; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands., Eikmans M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands., Ruben JM; Div. of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands., van Kooten C; Div. of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, the Netherlands. Electronic address: C.van_Kooten@lumc.nl. |
| مؤلفون مشاركون: | COMBAT consortium |
| المصدر: | Journal of immunological methods [J Immunol Methods] 2020 Apr; Vol. 479, pp. 112748. Date of Electronic Publication: 2020 Jan 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam, North-Holand, |
| مواضيع طبية MeSH: | Culture Media/*metabolism , Dendritic Cells/*immunology , T-Lymphocytes/*immunology, Cell Differentiation ; Cells, Cultured ; Culture Media/chemistry ; Humans ; Lymphocyte Activation ; RNA, Small Interfering/genetics ; Transfection |
| مستخلص: | Gene silencing using small interfering ribonucleic acids (siRNA) is a powerful method to interfere with gene expression, allowing for the functional exploration of specific genes. siRNA interference can be applied in both cell lines, as well as in primary, non-dividing cell types like dendritic cells. However, the efficacy in different cell types is variable and requires optimization. Here, we showed that the type of culture medium used during lipid-based siRNA-mediated transfection acts as a critical factor, affecting dendritic cell activation. Transfection of immature monocyte-derived dendritic cells in RPMI medium, but not in IMDM, showed increased transcript levels of pro-inflammatory cytokines. Moreover, the expression of co-stimulatory molecules was enhanced, thereby increasing the T cell stimulatory capacity. Our data demonstrates that the choice of medium should be critically examined as one of the variables while optimizing cell transfection. Competing Interests: Disclosure None. (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.) |
| التعليقات: | Comment in: Genes Immun. 2024 Feb;25(1):43-54. doi: 10.1038/s41435-023-00243-6. (PMID: 38146001) |
| فهرسة مساهمة: | Keywords: Cell maturation; Dendritic cells; RNA interference; Transfection; siRNA |
| المشرفين على المادة: | 0 (Culture Media) 0 (RNA, Small Interfering) |
| تواريخ الأحداث: | Date Created: 20200121 Date Completed: 20201119 Latest Revision: 20240625 |
| رمز التحديث: | 20240625 |
| DOI: | 10.1016/j.jim.2020.112748 |
| PMID: | 31958452 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7905 |
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| DOI: | 10.1016/j.jim.2020.112748 |