دورية أكاديمية
Simultaneous Derivation of Embryonic and Trophoblast Stem Cells from Mouse Blastocysts.
| العنوان: | Simultaneous Derivation of Embryonic and Trophoblast Stem Cells from Mouse Blastocysts. |
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| المؤلفون: | Kidder BL; Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA. Benjamin.kidder@wayne.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2117, pp. 235-241. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Blastocyst Inner Cell Mass/*cytology , Cell Culture Techniques/*methods , Embryonic Stem Cells/*cytology , Trophoblasts/*cytology, Animals ; Biomarkers ; Blastocyst Inner Cell Mass/metabolism ; Cell Differentiation ; Cell Self Renewal ; Culture Media/metabolism ; Embryonic Stem Cells/metabolism ; Epigenesis, Genetic ; Female ; Fibroblast Growth Factor 4/metabolism ; Mice ; Pregnancy ; Trophoblasts/metabolism |
| مستخلص: | The formation of the blastocyst during mammalian development involves the segregation of two populations of cells with unequal potential: pluripotent cells of the inner cell mass (ICM) and multipotent cells of the trophectoderm (TE). ICM cells maintain the capacity to give rise to all cells represented in the organism, while TE cells, which represent the first lineage to emerge during development, are capable of differentiating into trophoblast lineages of the placenta. The ICM and TE are both essential for development. The ICM is genetically programmed to generate all cells of the embryo proper, while the TE forms extraembryonic trophoblast lineages and is required for implantation of the embryo and maternal-fetal exchange of nutrients and waste. Embryonic stem (ES) cells, which can be derived from the ICM of blastocysts in the presence of external signals such as LIF, can self-renewal indefinitely, and because they can differentiate into all cells of the organism, ES cells are a widely used in vitro model to study genetics and development. Trophoblast stem (TS) cells can be derived from the TE of blastocyst stage embryos in the presence of FGF4, and like ES cells, TS cells are also able to self-renew indefinitely. Because TS cells can differentiate into epithelial lineages of the trophoblast, TS cells are an ideal in vitro model to study the biology of the trophoblast. In this chapter, we describe protocols for simultaneous derivation of ES cells and TS cells from mouse blastocysts and culture conditions that promote self-renewal of hybrid ESC/TSC colonies. These protocols are sufficient for efficient derivation of hybrid ESC/TSC colonies. |
| فهرسة مساهمة: | Keywords: Blastocyst; Derivation; Differentiation; Embryonic stem cells; Inner cell mass; Multipotent; Pluripotent; Trophectoderm; Trophoblast stem cells |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Culture Media) 0 (Fibroblast Growth Factor 4) |
| تواريخ الأحداث: | Date Created: 20200122 Date Completed: 20210208 Latest Revision: 20210208 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-0716-0301-7_14 |
| PMID: | 31960383 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-0301-7_14 |