دورية أكاديمية
Production and characterization of radiation-sensitive meiotic mutants of Coprinus cinereus.
| العنوان: | Production and characterization of radiation-sensitive meiotic mutants of Coprinus cinereus. |
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| المؤلفون: | Zolan ME; Department of Natural Sciences, University of Michigan-Dearborn 48128., Tremel CJ, Pukkila PJ |
| المصدر: | Genetics [Genetics] 1988 Oct; Vol. 120 (2), pp. 379-87. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0374636 Publication Model: Print Cited Medium: Print ISSN: 0016-6731 (Print) Linking ISSN: 00166731 NLM ISO Abbreviation: Genetics Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Austin, Tex. [etc.] |
| مواضيع طبية MeSH: | Mutation* , Radiation Tolerance*, Agaricales/*genetics , Coprinus/*genetics, Cobalt Radioisotopes ; Coprinus/cytology ; DNA Repair/radiation effects ; Genetic Complementation Test ; Ultraviolet Rays |
| مستخلص: | We have isolated four gamma-ray-sensitive mutants of the basidiomycete Coprinus cinereus. When homozygous, two of these (rad 3-1 and rad 9-1) produce fruiting bodies with very few viable basidiospores, the products of meiosis in this organism. A less radiation-sensitive allele of RAD 3, rad 3-2, causes no apparent meiotic defect in homozygous strains. Quantitative measurements of oidial survival of rad 3-1; rad 9-1 double mutants compared to the single mutants indicated that rad 3-1 and rad 9-1 mutants are defective in the same DNA repair pathway. In the few viable basidiospores that are produced by these two strains, essentially normal levels of meiotic recombination can be detected. None of the mutants exhibits increased sensitivity to UV radiation. Cytological examination of meiotic chromosomes from mutant and wild-type fruiting bodies showed that rad 3-1 homozygous strains fail to condense and pair homologous chromosomes during prophase I. Although rad 9-1 strains are successful at chromosome pairing, meiosis is usually not completed in these mutants. |
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| معلومات مُعتمدة: | CA07395 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Cobalt Radioisotopes) |
| تواريخ الأحداث: | Date Created: 19881001 Date Completed: 19890113 Latest Revision: 20210915 |
| رمز التحديث: | 20221208 |
| مُعرف محوري في PubMed: | PMC1203517 |
| DOI: | 10.1093/genetics/120.2.379 |
| PMID: | 3197952 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0016-6731 |
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| DOI: | 10.1093/genetics/120.2.379 |