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Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.

التفاصيل البيبلوغرافية
العنوان: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.
المؤلفون: Ast J; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Arvaniti A; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Fine NHF; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Nasteska D; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Ashford FB; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Stamataki Z; Centre for Liver Research, College of Medical and Dental Sciences, Institute for Immunology and Immunotherapy, University of Birmingham, Birmingham, UK., Koszegi Z; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Bacon A; Genome Editing Facility, Technology Hub, University of Birmingham, Birmingham, UK., Jones BJ; Division of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London, London, UK., Lucey MA; Division of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London, London, UK., Sasaki S; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia, Vancouver, BC, Canada., Brierley DI; Centre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London, London, UK., Hastoy B; Oxford Centre for Diabetes, Endocrinology & Metabolism, University of Oxford, Oxford, UK., Tomas A; Division of Diabetes, Endocrinology and Metabolism, Section of Cell Biology and Functional Genomics, Imperial College London, London, UK., D'Agostino G; Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester, UK., Reimann F; Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, UK., Lynn FC; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia, Vancouver, BC, Canada., Reissaus CA; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA., Linnemann AK; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA., D'Este E; Optical Microscopy Facility, Max Planck Institute for Medical Research, Heidelberg, Germany., Calebiro D; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK., Trapp S; Centre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London, London, UK., Johnsson K; Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany., Podewin T; Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. tom.podewin@mpimf-heidelberg.mpg.de., Broichhagen J; Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. johannes.broichhagen@mr.mpg.de., Hodson DJ; Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK. d.hodson@bham.ac.uk.; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK. d.hodson@bham.ac.uk.
المصدر: Nature communications [Nat Commun] 2020 Jan 24; Vol. 11 (1), pp. 467. Date of Electronic Publication: 2020 Jan 24.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Fluorescent Dyes*/chemical synthesis , Fluorescent Dyes*/chemistry, Glucagon-Like Peptide-1 Receptor/*metabolism , Microscopy, Fluorescence, Multiphoton/*methods, Amino Acid Sequence ; Animals ; Brain/metabolism ; Cell Line ; Glucagon-Like Peptide-1 Receptor/antagonists & inhibitors ; Glucagon-Like Peptide-1 Receptor/deficiency ; Glucagon-Like Peptide-1 Receptor/genetics ; HEK293 Cells ; Human Embryonic Stem Cells/metabolism ; Humans ; Islets of Langerhans/metabolism ; Mice ; Mice, Knockout ; Models, Molecular ; Molecular Structure ; Peptide Fragments/chemical synthesis ; Peptide Fragments/chemistry ; Peptide Fragments/genetics ; Signal Transduction ; Tissue Distribution
مستخلص: The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.
التعليقات: Erratum in: Nat Commun. 2020 Oct 9;11(1):5160. (PMID: 33037231)
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معلومات مُعتمدة: MR/S025618/1 United Kingdom MRC_ Medical Research Council; MR/N02589X/1 United Kingdom MRC_ Medical Research Council; MR/P009824/2 United Kingdom MRC_ Medical Research Council; MR/R010676/1 United Kingdom MRC_ Medical Research Council; United Kingdom WT_ Wellcome Trust; R01 DK095757 United States DK NIDDK NIH HHS; MR/K023667/1 United Kingdom MRC_ Medical Research Council; MC_UU_12012/3 United Kingdom MRC_ Medical Research Council; MC_UU_00014/3 United Kingdom MRC_ Medical Research Council; T32 DK064466 United States DK NIDDK NIH HHS; U24 DK104162 United States DK NIDDK NIH HHS; MR/N00275X/1 United Kingdom MRC_ Medical Research Council; P30 DK097512 United States DK NIDDK NIH HHS; MR/P009824/1 United Kingdom MRC_ Medical Research Council; MC_UU_00014/5 United Kingdom MRC_ Medical Research Council; U01 DK104162 United States DK NIDDK NIH HHS; R03 DK115990 United States DK NIDDK NIH HHS
المشرفين على المادة: 0 (Fluorescent Dyes)
0 (GLP1R protein, human)
0 (Glp1r protein, mouse)
0 (Glucagon-Like Peptide-1 Receptor)
0 (Peptide Fragments)
5313W10MYT (exendin (9-39))
تواريخ الأحداث: Date Created: 20200126 Date Completed: 20200413 Latest Revision: 20220417
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6981144
DOI: 10.1038/s41467-020-14309-w
PMID: 31980626
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-020-14309-w