دورية أكاديمية
Dynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solution.
| العنوان: | Dynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solution. |
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| المؤلفون: | Paterson KA; EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, United Kingdom., Arlt J, Jones AC |
| المصدر: | Methods and applications in fluorescence [Methods Appl Fluoresc] 2020 Feb 19; Vol. 8 (2), pp. 025002. Date of Electronic Publication: 2020 Feb 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: IOP Publishing Country of Publication: England NLM ID: 101608648 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-6120 (Electronic) Linking ISSN: 20506120 NLM ISO Abbreviation: Methods Appl Fluoresc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bristol : IOP Publishing, 2013- |
| مواضيع طبية MeSH: | Fluorescence*, 2-Aminopurine/*chemistry , DNA/*chemistry , Nucleotides/*chemistry |
| مستخلص: | 2-aminopurine (2AP) is a responsive fluorescent base analogue that is used widely as a probe of the local molecular environment in DNA. The ability of 2AP to report changes in local conformation and base-stacking interactions arises from the efficient quenching of its fluorescence by the natural DNA bases. However, the mechanism of this inter-base quenching remains imperfectly understood. Two previous studies of the collisional quenching of 2AP by the natural bases, in different buffer solutions, showed that dynamic quenching efficiency depends on the identity of the natural base, but disagreed on the relative quenching efficiencies of the bases. We report a comprehensive investigation of inter-base quenching of 2AP by the natural nucleoside monophosphates (NMPs), replicating the buffer conditions used in the previous studies. Using time-resolved fluorescence measurements to distinguish between dynamic and static quenching, we find that the dynamic quenching rate constants of the different bases show a consistent trend across both buffers, and this is in line with a charge-transfer mechanism. Time-resolved measurements also provide insight into static quenching, revealing formation of 2AP-NMP ground-state complexes in which 2AP displays a very short fluorescence lifetime, comparable to that seen in oligonucleotides. In these complexes, the dependence of the rate of quenching on the partner base also supports a charge-transfer mechanism. |
| المشرفين على المادة: | 0 (Nucleotides) 452-06-2 (2-Aminopurine) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20200131 Date Completed: 20201007 Latest Revision: 20201007 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1088/2050-6120/ab71c3 |
| PMID: | 32000159 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2050-6120 |
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| DOI: | 10.1088/2050-6120/ab71c3 |