دورية أكاديمية
Cell-Based Assays for Modeling Xenogeneic Immune Responses.
| العنوان: | Cell-Based Assays for Modeling Xenogeneic Immune Responses. |
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| المؤلفون: | Casós K; Infectious Diseases and Transplantation Division, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.; Department of Cardiac Surgery and Reparative Therapy of the Heart, Vall d'Hebron Research Institute (VHIR), University Hospital Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain., Sommaggio R; Infectious Diseases and Transplantation Division, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain., Pérez-Cruz M; Infectious Diseases and Transplantation Division, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.; Division of Blood and Marrow Transplantation, Stanford University Medical Center, Stanford, CA, USA.; Immunologie et NeurogÕnÕtique Expérimentales et MolÕculaires (INEM), Le Studium-CNRS, Orleans, France., Costa C; Infectious Diseases and Transplantation Division, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain. ccosta@idibell.cat. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2110, pp. 99-113. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Cell Culture Techniques* , Transplantation, Heterologous*/adverse effects , Transplantation, Heterologous*/methods, Antigens, Heterophile/*immunology , Biological Assay/*methods , Graft Rejection/*immunology , Heterografts/*immunology, Animals ; Coculture Techniques ; Cytokines/metabolism ; Cytotoxicity, Immunologic ; Graft Rejection/diagnosis ; Graft Rejection/metabolism ; Humans ; Killer Cells, Natural/immunology ; Killer Cells, Natural/metabolism ; Swine ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Transplantation Immunology |
| مستخلص: | Research in xenotransplantation implies a high experimental complexity comprising molecular, cellular, and in vivo studies to investigate the mechanisms of xenograft immune rejection and functional failure, as well as the strategies to counteract them. After major advances associated with the identification of the carbohydrate xenoantigens and their elimination through genomic edition of the source pigs, the study of the cellular immune response against the xenograft is gaining particular attention. Xenogeneic cell-based assays that put together pig cells and human leukocytes such as monocytes, NK cells, and T cells are relevant to address this hurdle. Thus, we describe here coculture, co-stimulatory, and cytotoxicity assays for investigating the cellular and molecular mechanisms of xenograft rejection. These techniques allow elucidating the key pathways that take place during the xenogeneic immune response in a simplified setting. Treatment with either pro-inflammatory or anti-inflammatory cytokines can be used for studying the regulation of adhesion, co-stimulatory molecules, and receptors involved in triggering the immune response under various conditions. Furthermore, these assays can be used for the follow-up of the immune response of in vivo studies as well as for the development of tolerogenic approaches that promote xenograft survival. |
| فهرسة مساهمة: | Keywords: Cytokines; Human; Immune response; Monocytes; NK cells; Pig; T cells |
| المشرفين على المادة: | 0 (Antigens, Heterophile) 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20200201 Date Completed: 20210127 Latest Revision: 20210127 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-0255-3_7 |
| PMID: | 32002904 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-0255-3_7 |