دورية أكاديمية
أعرض في EDS

Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species.

التفاصيل البيبلوغرافية
العنوان: Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species.
المؤلفون: da Silva AF; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., da Rocha CQ; Laboratório de Química de Produtos Naturais, Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., da Silva LCN; Laboratório de Imunologia das Doenças Infecciosas e Parasitárias, Programa de Mestrado em Biologia Microbiana, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil., Carvalho Júnior AR; Laboratório de Imunologia das Doenças Infecciosas e Parasitárias, Programa de Mestrado em Biologia Microbiana, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil., Mendes INFV; Departamento de Ensino, Instituto Federal do Maranhão, 65485-000 Itapecuru-Mirim, Ma, Brazil., de Araruna AB; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Motta EP; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Silva RS; Laboratório de Microbiologia Aplicada, Programa de Biologia Microbiana, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil., Campos CDL; Laboratório de Microbiologia Aplicada, Programa de Biologia Microbiana, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil., Farias JR; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Oliveira ADS; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Silva DHDS; Laboratório de Microbiologia Aplicada, Curso de Especialização em Microbiologia Clínica, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil., Nascimento FRF; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Guerra RNM; Laboratório de Imunofisiologia, Programa de Doutorado em Biotecnologia-RENORBIO, Universidade Federal do Maranhão, 65085-580 São Luís, Ma, Brazil., Monteiro CA; Laboratório de Microbiologia Aplicada, Programa de Biologia Microbiana, Universidade Ceuma, 65075-120 São Luís, Ma, Brazil.; Departamento de Biologia, Instituto Federal do Maranhão, 65030-005 São Luís, Ma, Brazil.
المصدر: Pathogens (Basel, Switzerland) [Pathogens] 2020 Jan 28; Vol. 9 (2). Date of Electronic Publication: 2020 Jan 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Print ISSN: 2076-0817 (Print) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Vulvovaginal candidiasis is a common fungal infection in women. In this study, Platonia insignis hydroalcoholic extract (PiHE) and its fractions were evaluated for antifungal and antivirulence activities against vaginal Candida species. Dichloromethane (DCMF) and ethyl acetate fractions (EAF) obtained from PiHE effectively inhibited the pathogen. Electrospray ionization mass spectrometry was used for identifying the main compounds in extracts. Minimal inhibitory and fungicidal concentrations (MIC and MFC, respectively) were determined by a broth microdilution assay. Furthermore, we evaluated the effect of the extract and fractions on the virulence properties of Candida albicans , and their cytotoxicity effect was determined on RAW 264.7 cells. Compounds found in extracts were flavonoid glycosides, mainly derivatives of quercetin and myricetin. Extracts showed antifungal potential, with the lowest MIC value for EAF (1.3 mg/mL) and inhibited Candida adherence and biofilm formation. EAF disrupted 48 h biofilms with an inhibition rate of more than 90%. The extract and its fractions exhibited no cytotoxicity. The antifungal effects were attributed to the ability of these extracts to alter the mitochondrial membrane potential for the release of pro-apoptotic factors in the cytosol. In conclusion, our data suggest that PiHE and EAF could act as novel candidates for the development of new therapeutic treatments against fungal infections.
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معلومات مُعتمدة: 311389/2016-3 Conselho Nacional de Desenvolvimento Científico e Tecnológico; PAEDT 03230/17 Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão
فهرسة مساهمة: Keywords: Candida albicans; Candida glabrata; Platonia insignis; antifungals; biofilm; vulvovaginal candidiasis
تواريخ الأحداث: Date Created: 20200205 Latest Revision: 20200928
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7168675
DOI: 10.3390/pathogens9020084
PMID: 32013047
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-0817
DOI:10.3390/pathogens9020084