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Culture dependent and independent analyses suggest a low level of sharing of endospore-forming species between mothers and their children.

التفاصيل البيبلوغرافية
العنوان: Culture dependent and independent analyses suggest a low level of sharing of endospore-forming species between mothers and their children.
المؤلفون: Avershina E; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1433, Ås, Norway. ekaterina.avershina@gmail.com.; Faculty of Applied Ecology, Agricultural Sciences and Biotechnology, Inland Norway University of Applied Sciences, 2316, Hamar, Norway. ekaterina.avershina@gmail.com., Larsen MG; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1433, Ås, Norway., Aspholm M; Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 369 Sentrum, 0102, Oslo, Norway., Lindback T; Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 369 Sentrum, 0102, Oslo, Norway., Storrø O; Department of Public Health and Nursing, Norwegian University of Science and Technology, 7491, Trondheim, Norway., Øien T; Department of Public Health and Nursing, Norwegian University of Science and Technology, 7491, Trondheim, Norway., Johnsen R; Department of Public Health and Nursing, Norwegian University of Science and Technology, 7491, Trondheim, Norway., Rudi K; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1433, Ås, Norway.
المصدر: Scientific reports [Sci Rep] 2020 Feb 04; Vol. 10 (1), pp. 1832. Date of Electronic Publication: 2020 Feb 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Gastrointestinal Microbiome*, Endospore-Forming Bacteria/*metabolism, Actinobacteria/genetics ; Bacteriological Techniques ; Child, Preschool ; Clostridiales/genetics ; Endospore-Forming Bacteria/genetics ; Feces/microbiology ; Female ; Humans ; Infant ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; RNA, Ribosomal, 16S/genetics ; Real-Time Polymerase Chain Reaction
مستخلص: Spore forming bacteria comprise a large part of the human gut microbiota. However, study of the endospores in gut microbiota is limited due to difficulties of culturing and numerous unknown germination factors. In this study we propose a new method for culture-independent characterization of endospores in stool samples. We have enriched DNA of spore-forming bacterial species from stool samples of 40 mother-child pairs from a previously described mother-child cohort. The samples were exposed to a two-step purification process comprising ethanol and ethidium monoazide (EMA) treatment to first kill vegetative cells and to subsequently eliminate their DNA from the samples. The composition of the ethanol-EMA resistant DNA was characterized by 16S rRNA marker gene sequencing. Operational taxonomic units (OTUs) belonging to the Clostridia class (OTU1: Romboutsia, OTU5: Peptostreptococcaceae and OTU14: Clostridium senso stricto) and one belonging to the Bacillus class (OTU20: Turicibacter) were significantly more abundant in the samples from mothers and children after ethanol-EMA treatment than in those treated with ethanol only. No correlation was observed between ethanol-EMA resistant OTUs detected in children and in their mothers, which indicates that a low level of spore-forming species are shared between mothers and their children. Anaerobic ethanol-resistant bacteria were isolated from all mothers and all children over 1 year of age. Generally, in 70% of the ethanol-treated samples used for anaerobic culturing, 16S rRNA gene sequences of bacterial isolates corresponded to OTUs detected in these samples after EMA treatment. We report a new DNA-based method for the characterization of endospores in gut microbiota. Our method has high degree of correspondence to the culture-based method, although it requires further optimization. Our results also indicate a high turnover of endospores in the gut during the first two years of life, perhaps with a high environmental impact.
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المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20200206 Date Completed: 20201110 Latest Revision: 20210203
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7000398
DOI: 10.1038/s41598-020-58858-y
PMID: 32020012
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-58858-y