دورية أكاديمية
Backbone assignment of ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB).
| العنوان: | Backbone assignment of ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB). |
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| المؤلفون: | Bartkevihi L; Institute of Medical Biochemistry (IBqM), National Center of Nuclear Magnetic Resonance Jiri Jonas, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; National Center of Nuclear Magnetic Resonance (CNRMN), Center for Structural Biology and Bioimaging (CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Martins B; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira DMP; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Pires JRM; Institute of Medical Biochemistry (IBqM), National Center of Nuclear Magnetic Resonance Jiri Jonas, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; National Center of Nuclear Magnetic Resonance (CNRMN), Center for Structural Biology and Bioimaging (CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Anobom CD; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. anobom@iq.ufrj.br., Almeida FCL; Institute of Medical Biochemistry (IBqM), National Center of Nuclear Magnetic Resonance Jiri Jonas, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. falmeida@bioqmed.ufrj.br.; National Center of Nuclear Magnetic Resonance (CNRMN), Center for Structural Biology and Bioimaging (CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. falmeida@bioqmed.ufrj.br. |
| المصدر: | Biomolecular NMR assignments [Biomol NMR Assign] 2020 Apr; Vol. 14 (1), pp. 119-122. Date of Electronic Publication: 2020 Feb 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 101472371 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1874-270X (Electronic) Linking ISSN: 1874270X NLM ISO Abbreviation: Biomol NMR Assign Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht : Springer |
| مواضيع طبية MeSH: | Nuclear Magnetic Resonance, Biomolecular*, Aldose-Ketose Isomerases/*chemistry , Mycobacterium tuberculosis/*enzymology, Protein Structure, Secondary |
| مستخلص: | Tuberculosis is one of the deadliest diseases worldwide affecting approximately 10 million people in 2018. This classifies tuberculosis as epidemic in several countries and leads to an increasing number of multidrug-resistant strains. Thus, the development of new drugs is essential to effective treatments. A potential drug target is the ribose-5-phosphate isomerase, a ubiquitous enzyme important to nucleotide and cofactor biosynthesis. Here, we report the backbone assignment of ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB) that has been performed by triple resonance sequential approach using a [ 13 C, 15 N, 2 H]-labeled protein. This is the first ribose-5-phosphate isomerase, an enzyme previously classified as highly druggable, to be assigned. These data will be important to further screening studies to find inhibitors and determine their interaction with MtRpiB. |
| References: | Capriles PVSZ, Baptista LPR, Guedes IA et al (2015) Structural modeling and docking studies of ribose 5-phosphate isomerase from Leishmania major and Homo sapiens: a comparative analysis for Leishmaniasis treatment. J Mol Graph Model 55:134–147. https://doi.org/10.1016/j.jmgm.2014.11.002. (PMID: 10.1016/j.jmgm.2014.11.002) DeJesus MA, Gerrick ER, Xu W et al (2017) Comprehensive essentiality analysis of the Mycobacterium tuberculosis genome via saturating transposon mutagenesis. MBio 8:1–17. https://doi.org/10.1128/mBio.02133-16. (PMID: 10.1128/mBio.02133-16) Delaglio F, Grzesiek S, Vuister G et al (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR. https://doi.org/10.1007/BF00197809. (PMID: 10.1007/BF00197809) Faria J, Loureiro I, Santarém N et al (2016) Disclosing the essentiality of ribose-5-phosphate isomerase B in Trypanosomatids. Sci Rep 6:26937. https://doi.org/10.1038/srep26937. (PMID: 10.1038/srep26937) Griesinger C, Sattler M (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Progr Nucl Magn Reson Spectrosc 34:93–158. (PMID: 10.1016/S0079-6565(98)00025-9) Hyberts SG, Milbradt AG, Wagner AB et al (2012) Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling. J Biomol NMR 52:315–327. https://doi.org/10.1007/s10858-012-9611-z. (PMID: 10.1007/s10858-012-9611-z) Roos AK, Andersson CE, Bergfors T et al (2004) Mycobacterium tuberculosis ribose-5-phosphate isomerase has a known fold, but a novel active site. J Mol Biol 335:799–809. https://doi.org/10.1016/j.jmb.2003.11.021. (PMID: 10.1016/j.jmb.2003.11.021) Roos AK, Burgos E, Ericsson DJ et al (2005) Competitive inhibitors of Mycobacterium tuberculosis ribose-5-phosphate isomerase B reveal new information about the reaction mechanism. J Biol Chem 280:6416–6422. https://doi.org/10.1074/jbc.M412018200. (PMID: 10.1074/jbc.M412018200) Shen Y, Bax A (2013) Protein backbone and sidechain torsion angles predicted from NMR chemical shifts using artificial neural networks. J Biomol NMR 56:227–241. https://doi.org/10.1007/s10858-013-9741-y. (PMID: 10.1007/s10858-013-9741-y) Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:213–223. https://doi.org/10.1007/s10858-009-9333-z. (PMID: 10.1007/s10858-009-9333-z) Sørensen KI, Hove-Jensen B (1996) Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression. J Bacteriol 178:1003–1011. (PMID: 10.1128/JB.178.4.1003-1011.1996) Tugarinov V, Kanelis V, Kay LE (2006) Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy. Nat Protoc 1:749–754. https://doi.org/10.1038/nprot.2006.101. (PMID: 10.1038/nprot.2006.101) Vranken WF, Boucher W, Stevens TJ et al (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins Struct Funct Bioinform 59:687–696. https://doi.org/10.1002/prot.20449. (PMID: 10.1002/prot.20449) World Health Organization (2019) Global tuberculosis report 2019. Global tuberculosis report 2019. |
| فهرسة مساهمة: | Keywords: Inhibition; Mycobacterium tuberculosis; NMR; Pentose phosphate pathway; RpiB |
| المشرفين على المادة: | EC 5.3.1.- (Aldose-Ketose Isomerases) EC 5.3.1.6 (ribosephosphate isomerase) |
| تواريخ الأحداث: | Date Created: 20200208 Date Completed: 20201217 Latest Revision: 20220625 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/s12104-020-09931-0 |
| PMID: | 32030620 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1874-270X |
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| DOI: | 10.1007/s12104-020-09931-0 |