دورية أكاديمية
Construction and heterologous overexpression of two chimeric proteins carrying outer hydrophilic loops of Vairimorpha ceranae and Nosema bombycis ATP/ADP carriers.
| العنوان: | Construction and heterologous overexpression of two chimeric proteins carrying outer hydrophilic loops of Vairimorpha ceranae and Nosema bombycis ATP/ADP carriers. |
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| المؤلفون: | Dolgikh VV; Laboratory of Molecular Plant Protection, All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia. Electronic address: dol1slav@yahoo.com., Timofeev SA; Laboratory of Molecular Plant Protection, All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia., Zhuravlyov VS; Laboratory of Molecular Plant Protection, All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia., Senderskiy IV; Laboratory of Molecular Plant Protection, All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia. |
| المصدر: | Journal of invertebrate pathology [J Invertebr Pathol] 2020 Mar; Vol. 171, pp. 107337. Date of Electronic Publication: 2020 Feb 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0014067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0805 (Electronic) Linking ISSN: 00222011 NLM ISO Abbreviation: J Invertebr Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Gene Expression*, Fungal Proteins/*genetics , Microsporidia/*genetics , Nosema/*genetics , Recombinant Fusion Proteins/*genetics, Fungal Proteins/chemistry ; Fungal Proteins/metabolism ; Microsporidia/chemistry ; Microsporidia/metabolism ; Mitochondrial ADP, ATP Translocases/chemistry ; Mitochondrial ADP, ATP Translocases/genetics ; Mitochondrial ADP, ATP Translocases/metabolism ; Nosema/chemistry ; Nosema/metabolism ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/metabolism |
| مستخلص: | Microsporidia Nosema bombycis and Vairimorpha ceranae cause destructive epizootics of honey bees and silkworms. Insufficient efficiency of the antibiotic fumagillin against V. ceranae, its toxicity and the absence of effective methods of N. bombycis treatment demand the discovery of novel strategies to suppress infections of domesticated insects. RNA interference is one such novel treatment strategy. Another one implies that the intracellular development of microsporidia may be suppressed by single-chain antibodies (scFv fragments) against functionally important parasite proteins. Important components of microsporidian metabolism are non-mitochondrial, plastidic-bacterial ATP/ADP carriers. These membrane transporters import host-derived ATP and provide the capacity to pathogens for energy parasitism. Here, we analyzed membrane topology of four V. ceranae and three N. bombycis ATP/ADP transporters to construct two fusion proteins carrying their outer hydrophilic loops contacting with infected host cell cytoplasm. Interestingly, full-size genes of N. bombycis transporters may be derived from the Asian swallowtail Papilio xuthus genome sequencing project. Synthesis of the artificial genes was followed by overexpression of recombinant proteins in E. coli as insoluble inclusion bodies. The gene fragments encoding the loops of individual transporters were also effectively expressed in bacteria. The chimeric antigens may be used to construct immune libraries or select microsporidia-suppressing scFv fragments from synthetic, semisynthetic, naïve and immune antibody libraries. A further expression of such antibodies in insect cells may increase their resistance to microsporidial infections. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibody libraries; Chimeric proteins; Domesticated insects; Heterologous expression; Microsporidiosis; Outer loops of ATP/ADP carriers |
| المشرفين على المادة: | 0 (Fungal Proteins) 0 (Recombinant Fusion Proteins) 9068-80-8 (Mitochondrial ADP, ATP Translocases) |
| SCR Organism: | Nosema bombycis; Vairimorpha cheracis |
| تواريخ الأحداث: | Date Created: 20200209 Date Completed: 20210429 Latest Revision: 20210429 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jip.2020.107337 |
| PMID: | 32035083 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0805 |
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| DOI: | 10.1016/j.jip.2020.107337 |