دورية أكاديمية
Single-Cell Transcriptome Atlas of Murine Endothelial Cells.
| العنوان: | Single-Cell Transcriptome Atlas of Murine Endothelial Cells. |
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| المؤلفون: | Kalucka J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., de Rooij LPMH; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Goveia J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Rohlenova K; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Dumas SJ; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Meta E; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Conchinha NV; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Taverna F; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Teuwen LA; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Veys K; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., García-Caballero M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Khan S; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Geldhof V; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Sokol L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Chen R; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Treps L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Borri M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., de Zeeuw P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Dubois C; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Karakach TK; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Falkenberg KD; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Parys M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Yin X; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Vinckier S; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Du Y; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Fenton RA; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark., Schoonjans L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Dewerchin M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Eelen G; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium., Thienpont B; Laboratory for Functional Epigenetics, Department of Human Genetics, KU Leuven, 3000 Leuven, Belgium., Lin L; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao, Shandong 266555, P.R. China., Bolund L; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao, Shandong 266555, P.R. China., Li X; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China. Electronic address: lixr6@mail.sysu.edu.cn., Luo Y; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao, Shandong 266555, P.R. China; BGI-Shenzhen, Shenzhen, Guangdong 518083, P.R. China; China National GeneBank, BGI-Shenzhen, Shenzhen, Guangdong 518120, P.R. China. Electronic address: alun@biomed.au.dk., Carmeliet P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, and Department of Oncology and Leuven Cancer Institute (LKI), VIB and KU Leuven, 3000 Leuven, Belgium; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China. Electronic address: peter.carmeliet@kuleuven.vib.be. |
| المصدر: | Cell [Cell] 2020 Feb 20; Vol. 180 (4), pp. 764-779.e20. Date of Electronic Publication: 2020 Feb 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مواضيع طبية MeSH: | Single-Cell Analysis* , Transcriptome*, Endothelial Cells/*metabolism, Animals ; Brain/cytology ; Cardiovascular System/cytology ; Endothelial Cells/classification ; Endothelial Cells/cytology ; Gastrointestinal Tract/cytology ; Male ; Mice ; Mice, Inbred C57BL ; Muscles/cytology ; Organ Specificity ; RNA-Seq ; Testis/cytology |
| مستخلص: | The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7 + intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. Arterial, venous, and lymphatic ECs shared more markers in more tissues than did heterogeneous capillary ECs. ECs from different vascular beds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarity across tissues, but the tissue (rather than the vessel) type contributed to the EC heterogeneity. Metabolic transcriptome analysis revealed a similar tissue-grouping phenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissues and between vascular beds within a single tissue in a tissue-type-dependent pattern. The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seq datasets and provides a powerful discovery tool and resource value. Competing Interests: Declaration of Interests None of the authors have competing financial interests to declare. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: endothelial metabolism; endothelial-cell heterogeneity; mouse endothelial atlas; single-cell RNA-seq |
| تواريخ الأحداث: | Date Created: 20200216 Date Completed: 20200824 Latest Revision: 20200824 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.cell.2020.01.015 |
| PMID: | 32059779 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4172 |
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| DOI: | 10.1016/j.cell.2020.01.015 |