دورية أكاديمية
Surveillance-embedded genomic outbreak resolution of methicillin-susceptible Staphylococcus aureus in a neonatal intensive care unit.
| العنوان: | Surveillance-embedded genomic outbreak resolution of methicillin-susceptible Staphylococcus aureus in a neonatal intensive care unit. |
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| المؤلفون: | Cremers AJH; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands. amelieke.cremers@radboudumc.nl., Coolen JPM; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., Bleeker-Rovers CP; Department of Internal Medicine, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., van der Geest-Blankert ADJ; Occupational Health & Safety and Environmental Service, Radboudumc, Nijmegen, the Netherlands., Haverkate D; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., Hendriks H; Department of Neonatology, Radboudumc, Nijmegen, the Netherlands., Henriet SSV; Department of Pediatrics, Radboudumc Amalia Children's Hospital, Nijmegen, the Netherlands., Huynen MA; Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands., Kolwijck E; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., Liem D; Department of Neonatology, Radboudumc, Nijmegen, the Netherlands., Melchers WJG; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., Rossen JW; Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands., Zoll J; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., van Heijst A; Department of Neonatology, Radboudumc, Nijmegen, the Netherlands., Hopman J; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands., Wertheim HFL; Department of Medical Microbiology, Radboudumc center for infectious diseases, Nijmegen, the Netherlands. |
| المصدر: | Scientific reports [Sci Rep] 2020 Feb 14; Vol. 10 (1), pp. 2619. Date of Electronic Publication: 2020 Feb 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Minisatellite Repeats*, Cross Infection/*epidemiology , Methicillin-Resistant Staphylococcus aureus/*genetics , Staphylococcal Infections/*epidemiology, Bacterial Typing Techniques ; Cross Infection/microbiology ; Cross Infection/transmission ; Cross-Sectional Studies ; Disease Outbreaks ; Humans ; Infant, Newborn ; Intensive Care Units, Neonatal ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Molecular Epidemiology ; Polymorphism, Single Nucleotide ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/transmission ; Whole Genome Sequencing |
| مستخلص: | We observed an increase in methicillin-susceptible Staphylococcus aureus (MSSA) infections at a Dutch neonatal intensive care unit. Weekly neonatal MSSA carriage surveillance and cross-sectional screenings of health care workers (HCWs) were available for outbreak tracing. Traditional clustering of MSSA isolates by spa typing and Multiple-Locus Variable number tandem repeat Analysis (MLVA) suggested that nosocomial transmission had contributed to the infections. We investigated whether whole-genome sequencing (WGS) of MSSA surveillance would provide additional evidence for transmission. MSSA isolates from neonatal infections, carriage surveillance, and HCWs were subjected to WGS and bioinformatic analysis for identification and localization of high-quality single nucleotide polymorphisms, and in-depth analysis of subsets of isolates. By measuring the genetic diversity in background surveillance, we defined transmission-level relatedness and identified isolates that had been unjustly assigned to clusters based on MLVA, while spa typing was concordant but of insufficient resolution. Detailing particular subsets of isolates provided evidence that HCWs were involved in multiple outbreaks, yet it alleviated concerns about one particular HCW. The improved resolution and accuracy of genomic outbreak analyses substantially altered the view on outbreaks, along with apposite measures. Therefore, inclusion of the circulating background population has the potential to overcome current issues in genomic outbreak inference. |
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| تواريخ الأحداث: | Date Created: 20200216 Date Completed: 20201113 Latest Revision: 20210213 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7021795 |
| DOI: | 10.1038/s41598-020-59015-1 |
| PMID: | 32060342 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-020-59015-1 |