دورية أكاديمية
Anti-genotoxic and anti-mutagenic effects of melatonin supplementation in a mouse model of melanoma.
| العنوان: | Anti-genotoxic and anti-mutagenic effects of melatonin supplementation in a mouse model of melanoma. |
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| المؤلفون: | Martins Longaretti L; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Luciano JA; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Strapazzon G; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Pereira M; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Damiani AP; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Rohr P; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Rigo FK; Laboratório de Fisiopatologia Exprimental, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., de Oliveira CA; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Steiner BT; Laboratório de Fisiopatologia Exprimental, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Vilela TC; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil., Trevisan G; Laboratory of Neuropsychopharmacology and Neurotoxicity, Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, Brazil., de Andrade VM; Laboratório de Biomedicina Translacional, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Brazil. |
| المصدر: | Drug and chemical toxicology [Drug Chem Toxicol] 2022 Mar; Vol. 45 (2), pp. 515-522. Date of Electronic Publication: 2020 Feb 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Group Country of Publication: United States NLM ID: 7801723 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-6014 (Electronic) Linking ISSN: 01480545 NLM ISO Abbreviation: Drug Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Philadelphia, PA] : Taylor & Francis Group Original Publication: New York, Dekker. |
| مواضيع طبية MeSH: | Antimutagenic Agents*/pharmacology , Melanoma* , Melatonin*/pharmacology, Animals ; Comet Assay ; DNA Damage ; Dietary Supplements ; Male ; Mice ; Mice, Inbred C57BL |
| مستخلص: | Melanoma, an aggressive skin cancer originating from melanocytes, can metastasize to the lungs, liver, cortex, femur, and spinal cord, ultimately resulting in DNA mutagenic effects. Melatonin is an endogenous hormone and free radical scavenger that possesses the ability to protect the DNA and to exert anti-proliferative effects in melanoma cells. The aim of this study was to evaluate the effects of B16F10 melanoma cells and the effects of melatonin supplementation on genotoxic parameters in murine melanoma models. Thirty-two male C57Bl/6 mice were divided in the following four groups: PBS + vehicle ( n = 6), melanoma + vehicle ( n = 10), PBS + melatonin ( n = 6), and melanoma + melatonin ( n = 10). The melanoma groups received a B16F10 cell injection, and melatonin was administered during 60 days. After treatment, tumor sizes were evaluated. DNA damage within the peripheral blood, lungs, liver, cortex, and spinal cord was determined using comet assay, and the mutagenicity within the bone marrow was determined using the micronucleus test. B16F10 cells effectively induced DNA damage in all tissues, and melatonin supplementation decreased DNA damage in the blood, liver, cortex, and spinal cord. This hormone exerts anti-tumor activity via its anti-proliferative, antioxidative, and pro-apoptotic effects. As this result was not observed within the lungs, we hypothesized that melatonin can induce apoptosis in cancer cells, and this was not evaluated by comet assay. This study provides evidence that melatonin can reduce the genotoxicity and mutagenicity caused by B16F10 cells. |
| فهرسة مساهمة: | Keywords: B16F10 cells; DNA damage; Melanoma; comet assay; genotoxicity; melatonin; micronucleus test; mutagenicity; skin cancer |
| المشرفين على المادة: | 0 (Antimutagenic Agents) JL5DK93RCL (Melatonin) |
| تواريخ الأحداث: | Date Created: 20200218 Date Completed: 20220223 Latest Revision: 20220223 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/01480545.2020.1726380 |
| PMID: | 32063063 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1525-6014 |
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| DOI: | 10.1080/01480545.2020.1726380 |