Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2 , for Cadmium Phytoremediation.

التفاصيل البيبلوغرافية
العنوان:	Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2 , for Cadmium Phytoremediation.
المؤلفون:	Tsyganov VE; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia.; Saint Petersburg Scientific Center (RAS), Saint Petersburg, Russia., Tsyganova AV; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Gorshkov AP; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Seliverstova EV; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia.; Sechenov Institute of Evolutionary Physiology and Biochemistry (RAS), Saint Petersburg, Russia., Kim VE; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Chizhevskaya EP; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Belimov AA; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Serova TA; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Ivanova KA; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Kulaeva OA; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Kusakin PG; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Kitaeva AB; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia., Tikhonovich IA; All-Russian Research Institute for Agricultural Microbiology, Saint Petersburg, Russia.; Department of Genetics and Biotechnology, Saint Petersburg State University, Saint Petersburg, Russia.
المصدر:	Frontiers in microbiology [Front Microbiol] 2020 Jan 29; Vol. 11, pp. 15. Date of Electronic Publication: 2020 Jan 29 (Print Publication: 2020).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة:	Original Publication: Lausanne : Frontiers Research Foundation
مستخلص:	Two transgenic strains of Rhizobium leguminosarum bv. viciae , 3841-PsMT1 and 3841-PsMT2, were obtained. These strains contain the genetic constructions nifH-PsMT1 and nifH-PsMT2 coding for two pea ( Pisum sativum L.) metallothionein genes, PsMT1 and PsMT2 , fused with the promoter region of the nifH gene. The ability of both transgenic strains to form nodules on roots of the pea wild-type SGE and the mutant SGECd ^t , which is characterized by increased tolerance to and accumulation of cadmium (Cd) in plants, was analyzed. Without Cd treatment, the wild type and mutant SGECd ^t inoculated with R. leguminosarum strains 3841, 3841-PsMT1, or 3841-PsMT2 were similar histologically and in their ultrastructural organization of nodules. Nodules of wild-type SGE inoculated with strain 3841 and exposed to 0.5 μM CdCl 2 were characterized by an enlarged senescence zone. It was in stark contrast to Cd-treated nodules of the mutant SGECd ^t that maintained their proper organization. Cadmium treatment of either wild-type SGE or mutant SGECd ^t did not cause significant alterations in histological organization of nodules formed by strains 3841-PsMT1 and 3841-PsMT2. Although some abnormalities were observed at the ultrastructural level, they were less pronounced in the nodules of strain 3841-PsMT1 than in those formed by 3841-PsMT2. Both transgenic strains also differed in their effects on pea plant growth and the Cd and nutrient contents in shoots. In our opinion, combination of Cd-tolerant mutant SGECd ^t and the strains 3841-PsMT1 or 3841-PsMT2 may be used as an original model for study of Cd tolerance mechanisms in legume-rhizobial symbiosis and possibilities for its application in phytoremediation or phytostabilization technologies. (Copyright © 2020 Tsyganov, Tsyganova, Gorshkov, Seliverstova, Kim, Chizhevskaya, Belimov, Serova, Ivanova, Kulaeva, Kusakin, Kitaeva and Tikhonovich.)
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فهرسة مساهمة:	Keywords: Rhizobium; bacteroid; cadmium tolerance; metallothionein; nodulation; symbiosome; symbiotic engineering
تواريخ الأحداث:	Date Created: 20200218 Latest Revision: 20240328
رمز التحديث:	20240329
مُعرف محوري في PubMed:	PMC7000653
DOI:	10.3389/fmicb.2020.00015
PMID:	32063892
قاعدة البيانات:	MEDLINE

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تدمد:	1664-302X
DOI:	10.3389/fmicb.2020.00015