دورية أكاديمية
Dynamin 2-dependent endocytosis is essential for mouse oocyte development and fertility.
العنوان: | Dynamin 2-dependent endocytosis is essential for mouse oocyte development and fertility. |
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المؤلفون: | Mihalas BP; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia., Redgrove KA; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia., Bernstein IR; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia., Robertson MJ; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre in Chemical Biology, University of Newcastle, Callaghan, NSW, Australia., McCluskey A; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre in Chemical Biology, University of Newcastle, Callaghan, NSW, Australia., Nixon B; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia., Holt JE; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; School of Biomedical Sciences & Pharmacy, University of Newcastle, Callaghan, NSW, Australia., McLaughlin EA; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia.; School of Science, Western Sydney University, Penrith, NSW, Australia.; School of Biological Sciences, The University of Auckland, Auckland, New Zealand., Sutherland JM; Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.; Pregnancy and Reproduction Program, Hunter Medial Research Institute, New Lambton Heights, NSW, Australia.; School of Biomedical Sciences & Pharmacy, University of Newcastle, Callaghan, NSW, Australia. |
المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2020 Apr; Vol. 34 (4), pp. 5162-5177. Date of Electronic Publication: 2020 Feb 17. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley Original Publication: [Bethesda, Md.] : The Federation, [c1987- |
مواضيع طبية MeSH: | Endocytosis* , Fertility*, Dynamin I/*physiology , Dynamin II/*physiology , Oocytes/*cytology , Ovarian Follicle/*cytology, Animals ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Oocytes/physiology ; Ovarian Follicle/physiology |
مستخلص: | During folliculogenesis, oocytes are dependent on metabolic and molecular support from surrounding somatic cells. Here, we examined the role of the dynamin (DNM) family of mechanoenzymes in mediating endocytotic uptake into growing follicular oocytes. We found DNM1 and DNM2 to be highly expressed in growing follicular oocytes as well as in mature germinal vesicle (GV) and metaphase II (MII) stage oocytes. Moreover, oocyte-specific conditional knockout (cKO) of DNM2 (DNM2Δ) led to complete sterility, with follicles arresting at the preantral stage of development. In addition, DNM2Δ ovaries were characterized by disrupted follicular growth as well as oocyte and follicle apoptosis. Further, the loss of DNM activity, either through DNM2 cKO or through pharmacological inhibition (Dyngo 6a) led to the impairment of endocytotic pathways in preantral oocytes as well as in mature GV and MII oocytes, respectively. Loss of DNM activity resulted in the redistribution of endosomes and the misslocalization of clathrin and actin, suggesting dysfunctional endocytosis. Notably, there was no observable effect on the fertility of DNM1Δ females. Our study has provided new insight into the complex and dynamic nature of oocyte growth during folliculogenesis, suggesting a role for DNM2 in mediating the endocytotic events that are essential for oocyte development. (© 2020 University of Newcastle. The FASEB Journal published by Wiley Periodicals, Inc. © 2020 Federation of American Societies for Experimental Biology.) |
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فهرسة مساهمة: | Keywords: dynamin; endocytosis; folliculogenesis; oocyte; oogenesis |
المشرفين على المادة: | EC 3.5.1.50 (Dynamin I) EC 3.6.5.5 (DNM2 protein, mouse) EC 3.6.5.5 (Dynamin II) |
تواريخ الأحداث: | Date Created: 20200218 Date Completed: 20210119 Latest Revision: 20210119 |
رمز التحديث: | 20231215 |
DOI: | 10.1096/fj.201902184R |
PMID: | 32065700 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1530-6860 |
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DOI: | 10.1096/fj.201902184R |