دورية أكاديمية
A comparison of prostate cancer cell transcriptomes in 2D monoculture vs 3D xenografts identify consistent gene expression alterations associated with tumor microenvironments.
| العنوان: | A comparison of prostate cancer cell transcriptomes in 2D monoculture vs 3D xenografts identify consistent gene expression alterations associated with tumor microenvironments. |
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| المؤلفون: | Brady L; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington., Gil da Costa RM; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington., Coleman IM; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington., Matson CK; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington., Risk MC; Department of Urology, University of Minnesota, Minneapolis, Minnesota., Coleman RT; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington., Nelson PS; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington.; Department of Medicine, University of Washington, Seattle, Washington. |
| المصدر: | The Prostate [Prostate] 2020 May; Vol. 80 (6), pp. 491-499. Date of Electronic Publication: 2020 Feb 18. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8101368 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0045 (Electronic) Linking ISSN: 02704137 NLM ISO Abbreviation: Prostate Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005-> : Hoboken, NJ : Wiley-Liss Original Publication: New York : Alan R. Liss, c1980- |
| مواضيع طبية MeSH: | Prostatic Neoplasms/*genetics , Prostatic Neoplasms/*pathology, Animals ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Genome, Human ; Heterografts ; Humans ; Immunohistochemistry ; Male ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Oligonucleotide Array Sequence Analysis/methods ; Transcriptome ; Tumor Cells, Cultured ; Tumor Microenvironment/genetics |
| مستخلص: | Background: Prostate cancer (PC) research has relied heavily on patient-derived cell lines, which may be used for in vitro (two-dimensional [2D]) studies or cultivated as three-dimensional (3D) xenografts in mice. These approaches are likely to have differential impacts on cell phenotypes, with implications for experimental outcomes. Therefore, defining and comparing the transcriptional signatures associated with 2D and 3D approaches may be useful for designing experiments and interpreting research results. Methods: In this study, LNCaP, VCaP, and 22Rv1 human PC cells were either cultivated in monolayers or as xenografts in NOD SCID mice, and their gene transcription profiles were quantitated and compared using microarray and real-time polymerase chain reaction techniques. Immunohistochemistry was used to evaluate protein expression in cancer cell xenografts. Results: Comparisons of gene expression profiles of tumor cells grown in 2D vs 3D environments identified gene sets featuring similar expression patterns in all three cancer cell lines and unique transcriptional signatures associated with 3D vs 2D growth. Pathways related to cell-cell interactions, differentiation, and the extracellular matrix were enriched in 3D conditions. Immunohistochemical analyses confirmed that gene upregulation in xenografts occurred in implanted cancer cells and not in mouse stromal cells. Cultivating cells in vitro in the presence of mouse, rather than bovine serum failed to elicit the gene transcription profile observed in xenografts, further supporting the hypothesis that this profile reflects 3D growth and enhanced microenvironmental interactions, rather than exposure to species-specific serum factors. Conclusions: Overall, these findings define the expression profiles observed in PC cells cultivated in 2D monolayers and in 3D xenografts, highlighting differentially regulated pathways in each setting and providing information for interpreting research results in model systems. (© 2020 Wiley Periodicals, Inc.) |
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| معلومات مُعتمدة: | P50CA097186 United States CA NCI NIH HHS; S10 OD020069 United States OD NIH HHS; P01 CA163227 United States CA NCI NIH HHS; P01CA163227 United States CA NCI NIH HHS; P50 CA097186 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: 3D model; in vitro; prostate cancer; transcriptome; xenograft |
| تواريخ الأحداث: | Date Created: 20200219 Date Completed: 20200715 Latest Revision: 20210502 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7148119 |
| DOI: | 10.1002/pros.23963 |
| PMID: | 32068909 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-0045 |
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| DOI: | 10.1002/pros.23963 |