دورية أكاديمية
The changing epidemiology of genital ulcer disease in South Africa: has donovanosis been eliminated?
| العنوان: | The changing epidemiology of genital ulcer disease in South Africa: has donovanosis been eliminated? |
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| المؤلفون: | Muller EE; STI Section, Centre for HIV & STIs, National Institute for Communicable Diseases, Johannesburg, South Africa etiennem@nicd.ac.za., Kularatne R; STI Section, Centre for HIV & STIs, National Institute for Communicable Diseases, Johannesburg, South Africa.; Department of Clinical Microbiology & Infectious Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. |
| المصدر: | Sexually transmitted infections [Sex Transm Infect] 2020 Dec; Vol. 96 (8), pp. 596-600. Date of Electronic Publication: 2020 Feb 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BMJ Pub. Group Country of Publication: England NLM ID: 9805554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1472-3263 (Electronic) Linking ISSN: 13684973 NLM ISO Abbreviation: Sex Transm Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BMJ Pub. Group, c1998- |
| مواضيع طبية MeSH: | Genital Diseases, Female/*microbiology , Genital Diseases, Male/*microbiology , Granuloma Inguinale/*microbiology, Adult ; Disease Eradication ; Female ; Genital Diseases, Female/diagnosis ; Genital Diseases, Female/epidemiology ; Genital Diseases, Male/diagnosis ; Genital Diseases, Male/epidemiology ; Granuloma Inguinale/diagnosis ; Granuloma Inguinale/epidemiology ; Humans ; Klebsiella/genetics ; Klebsiella/isolation & purification ; Klebsiella/physiology ; Male ; South Africa/epidemiology ; Ulcer ; Young Adult |
| مستخلص: | Objectives: We used an in-house molecular assay for the detection of Klebsiella granulomatis in ulcer specimens collected over a 12-year surveillance period in order to determine whether a diagnosis of donovanosis could be ascribed to genital ulcer disease (GUD) of unknown aetiology in our setting. Methods: Between 2007 and 2018, a total of 974 genital ulcer specimens with no previously identified sexually transmitted (STI) pathogens were selected from STI aetiological surveys conducted in all nine provinces of South Africa. Giemsa-stained ulcer smears from the same participants had previously been routinely analysed for the presence of typical Donovan bodies within large mononuclear cells. A Klebsiella screening assay targeting the phoE (phosphate porin) gene was used in combination with restriction digest analysis and sequencing to confirm the presence of K. granulomatis . Results: The Klebsiella screening assay tested positive in 19/974 (2.0%) genital ulcer specimens. Restriction digest analysis and nucleotide sequencing of the phoE gene confirmed that none of these specimens was positive for K. granulomatis DNA. Similarly, Donovan bodies were not identified in the Giemsa stained ulcer smears of these specimens. Conclusions: This is the first study to assess K. granulomatis as a cause of genital ulceration in South Africa over a 12-year surveillance period using molecular methods. The results demonstrate that K. granulomatis is no longer a prevalent cause of GUD in our population. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.) |
| فهرسة مساهمة: | Keywords: Africa; donovanosis; genital ulcers; granuloma inguinale |
| تواريخ الأحداث: | Date Created: 20200221 Date Completed: 20210218 Latest Revision: 20210218 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1136/sextrans-2019-054316 |
| PMID: | 32075875 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1472-3263 |
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| DOI: | 10.1136/sextrans-2019-054316 |