دورية أكاديمية
Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil.
| العنوان: | Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil. |
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| المؤلفون: | Murata FHA; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Previato M; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Frederico FB; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil.; Ambulatório de Oftalmologia Do Hospital de Base, Fundação Faculdade Regional de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil., Barbosa AP; Ambulatório de Oftalmologia Do Hospital de Base, Fundação Faculdade Regional de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil., Nakashima F; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil., de Faria GM Jr; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Silveira Carvalho AP; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Meira Strejevitch CDS; Laboratório de Biologia Molecular de Parasitas e Fungos Do Centro de Parasitologia e Micologia, Instituto Adolfo Lutz, São Paulo, Brazil., Pereira-Chioccola VL; Laboratório de Biologia Molecular de Parasitas e Fungos Do Centro de Parasitologia e Micologia, Instituto Adolfo Lutz, São Paulo, Brazil., Castiglioni L; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil., de Mattos LC; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Siqueira RC; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil., Brandão de Mattos CC; Faculdade de Medicina de São José Do Rio Preto, São José Do Rio Preto, Brazil.; FAMERP Toxoplasma Research Group, São José Do Rio Preto, Brazil. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2020 Feb 07; Vol. 9, pp. 472. Date of Electronic Publication: 2020 Feb 07 (Print Publication: 2019). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | Public Health*, Molecular Diagnostic Techniques/*methods , Serologic Tests/*methods , Toxoplasma/*genetics , Toxoplasma/*isolation & purification , Toxoplasmosis, Ocular/*diagnosis , Uveitis/*diagnosis, Antibodies, Protozoan/blood ; Brazil ; DNA, Protozoan/isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Ophthalmology ; Predictive Value of Tests ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Toxoplasma/immunology ; Toxoplasmosis, Ocular/parasitology ; Uveitis/parasitology |
| مستخلص: | Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii . The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial methods can aid to the correct diagnosis of this infection. We compared serological methods ELISA and ELFA, and molecular cPCR, Nested PCR and qPCR for the diagnosis of T. gondii infection in groups of patients clinically evaluated with ocular diseases non-toxoplasma related (G1 = 185) and with lesions caused by toxoplasmosis (G2 = 164) in an Ophthalmology clinic in Brazil. Results were compared by the Kappa index, and sensitivity (S), specificity (E), positive predictive value (PPV), and negative (NPV) were calculated. Serologic methods were in agreement with ELISA more sensitive and ELFA more specific to characterize the acute and chronic infections while molecular methods were discrepant where qPCR presented higher sensitivity, however, lower specificity when compared to cPCR and Nested PCR. (Copyright © 2020 Murata, Previato, Frederico, Barbosa, Nakashima, Faria, Silveira Carvalho, Meira Strejevitch, Pereira-Chioccola, Castiglioni, de Mattos, Siqueira and Brandão de Mattos.) |
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| فهرسة مساهمة: | Keywords: Toxoplasama gondii; ocular toxoplasmosis; polimerase chain reaction (PCR); qPCR; retinochoroiditis; toxoplasma antibodies; uveites |
| المشرفين على المادة: | 0 (Antibodies, Protozoan) 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20200303 Date Completed: 20200901 Latest Revision: 20200901 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7019025 |
| DOI: | 10.3389/fcimb.2019.00472 |
| PMID: | 32117820 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2235-2988 |
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| DOI: | 10.3389/fcimb.2019.00472 |