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The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad-snouted caiman (Caiman latirostris).

التفاصيل البيبلوغرافية
العنوان: The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad-snouted caiman (Caiman latirostris).
المؤلفون: Fonseca LA; Professor of Veterinary Clinical Pathology in Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Dornelas LRSM; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Orozco AMO; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Souto PC; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Bento LD; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Nóbrega YC; Projeto Caiman, Marcos Daniel Institute, Vitória, Minas Gerais, Brazil., Santos MRD; Projeto Caiman, Marcos Daniel Institute, Vitória, Minas Gerais, Brazil., Carvalho Filho WP; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Ermita PAN; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil., Girardi FM; Veterinary Department, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
المصدر: Veterinary clinical pathology [Vet Clin Pathol] 2020 Mar; Vol. 49 (1), pp. 59-65. Date of Electronic Publication: 2020 Mar 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Veterinary Clinical Pathology Country of Publication: United States NLM ID: 9880575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-165X (Electronic) Linking ISSN: 02756382 NLM ISO Abbreviation: Vet Clin Pathol Subsets: MEDLINE
أسماء مطبوعة: Publication: 1998- : Baton Rouge, LA : American Society for Veterinary Clinical Pathology
Original Publication: 1977-<1989> : Columbia, MO : Veterinary Practice Pub. Co.
مواضيع طبية MeSH: Alligators and Crocodiles/*blood , Blood Proteins/*analysis , Electrophoresis, Polyacrylamide Gel/*veterinary, Animals ; Ecosystem ; Female ; Male ; Sensitivity and Specificity ; Sex Factors
مستخلص: Background: Knowledge of mammalian inflammatory responses is vast; however, many aspects of the inflammatory response in non-mammalian vertebrates, such as reptiles, remain unclear, including those regarding acute-phase proteins (APPs). Recent studies have focused on the use of serum protein electrophoresis (SPE) to assess inflammatory responses in the broad-snouted caiman (Caiman latirostris) and other reptiles.
Objectives: The purpose of this study was to examine the effects of sex, body length, and different habitats on SPE patterns in C latirostris using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Methods: A total of 40 animals of both sexes and varying body lengths were collected; of these, 23 were free-living in an industrial complex (site 1), and 17 were captive on a rural property (site 2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed to fractionate different serum protein constituents.
Results: Sex affected protein levels, with females showing higher serum levels of total protein, as well as the 90-, 66-, and 58-kDa proteins compared with males. Similarly, body length affected protein levels, with male adults >1.70 m in length showing the lowest serum levels of 152-, 58-, 54-, and 27-kDa proteins of all other animals. Finally, habitat affected protein levels, with animals at site 1 showing higher serum levels of 152- and 41-kDa proteins compared with those at site 2.
Conclusions: This preliminary study was the first to evaluate the SPE of C latirostris using SDS-PAGE. Further studies to identify the proteins in each band with more specific and sensitive techniques (eg, mass spectrometry) should be conducted to elucidate the standard of APPs in reptiles better.
(© 2020 American Society for Veterinary Clinical Pathology.)
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معلومات مُعتمدة: Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
فهرسة مساهمة: Keywords: SDS-PAGE; protein; reptiles; serum biochemistry
المشرفين على المادة: 0 (Blood Proteins)
تواريخ الأحداث: Date Created: 20200305 Date Completed: 20210125 Latest Revision: 20210125
رمز التحديث: 20221213
DOI: 10.1111/vcp.12827
PMID: 32128863
قاعدة البيانات: MEDLINE
الوصف
تدمد:1939-165X
DOI:10.1111/vcp.12827