دورية أكاديمية
Andiroba oil (Carapa guianensis Aubl) shows cytotoxicity but no mutagenicity in the ACPP02 gastric cancer cell line.
| العنوان: | Andiroba oil (Carapa guianensis Aubl) shows cytotoxicity but no mutagenicity in the ACPP02 gastric cancer cell line. |
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| المؤلفون: | Porfírio-Dias CL; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Melo KM; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Bastos CEMC; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Ferreira TAA; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Azevedo LFC; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Salgado HL; Laboratório de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará,, Belém, Pará, Brazil., Santos AS; Laboratório de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará,, Belém, Pará, Brazil., Rissino JD; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Nagamachi CY; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil., Pieczarka JC; Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil. |
| المصدر: | Journal of applied toxicology : JAT [J Appl Toxicol] 2020 Aug; Vol. 40 (8), pp. 1060-1066. Date of Electronic Publication: 2020 Mar 09. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley And Sons Country of Publication: England NLM ID: 8109495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1263 (Electronic) Linking ISSN: 0260437X NLM ISO Abbreviation: J Appl Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Philadelphia, Pa. : Heyden & Son, c1981- |
| مواضيع طبية MeSH: | Apoptosis/*drug effects , Cytotoxicity, Immunologic/*drug effects , Cytotoxins/*toxicity , Meliaceae/*toxicity , Mutagenesis/*drug effects , Mutagens/*toxicity , Plant Oils/*toxicity , Stomach Neoplasms/*drug therapy, Antineoplastic Agents/therapeutic use ; Brazil ; Cells, Cultured/drug effects ; Humans ; Meliaceae/chemistry ; Plant Oils/chemistry ; Plants, Medicinal/chemistry ; Plants, Medicinal/toxicity |
| مستخلص: | Andiroba (Carapa guianensis Aubl) is an Amazonian plant whose oil has been widely used in traditional medicine for various purposes, including anti-inflammation. Research reports indicate that the oil can confer antitumor activity due to the presence of fatty acids, which can directly influence cell death mechanisms. Thus, andiroba oil (AO) has gained interest for its potential to be used in antineoplastic therapies. Here, we report an in vitro analysis of the cytotoxic and mutagenic potential of AO in the gastric cancer cell line, ACP02. Cell survival was assessed by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, differential staining with ethidium bromide and acridine orange assessed apoptosis-necrosis, and mutagenesis was assessed by the micronucleus test. The apolar oil was first diluted in 0.1% dimethyl sulfoxide (DMSO) and then further diluted to six concentrations (0.01, 0.1, 1, 10 and 100 μg/mL and 1 mg/mL) in RPMI medium. Controls included RPMI alone (negative control) and 0.1% DMSO diluted in medium (vehicle control). The MTT test showed that AO significantly reduced cell viability (P < .05) only when the highest tested concentration was applied for 48 hours. The apoptosis/necrosis test showed that the highest concentration of AO induced cell death by apoptosis at 24 and 48 hours. There was no statistically significant increase in the frequency of micronuclei. The ability of the AO to decrease the viability of ACP02 cells via apoptosis, without exerting mutagenic effects, suggests that the oil could be useful as an alternative therapeutic agent for primary tumors of stomach cancer. (© 2020 John Wiley & Sons, Ltd.) |
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| فهرسة مساهمة: | Keywords: Amazon biodiversity; apoptosis; micronucleus; phytotherapy; toxicity |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (Cytotoxins) 0 (Mutagens) 0 (Plant Oils) |
| تواريخ الأحداث: | Date Created: 20200310 Date Completed: 20211012 Latest Revision: 20211012 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jat.3966 |
| PMID: | 32150766 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-1263 |
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| DOI: | 10.1002/jat.3966 |