دورية أكاديمية
أعرض في EDS

Alterations of redox and iron metabolism accompany the development of HIV latency.

التفاصيل البيبلوغرافية
العنوان: Alterations of redox and iron metabolism accompany the development of HIV latency.
المؤلفون: Shytaj IL; Heidelberg University Hospital, Heidelberg, Germany.; German Center for Infection Research, Heidelberg, Germany., Lucic B; Heidelberg University Hospital, Heidelberg, Germany.; German Center for Infection Research, Heidelberg, Germany., Forcato M; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy., Penzo C; Heidelberg University Hospital, Heidelberg, Germany., Billingsley J; Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA., Laketa V; Heidelberg University Hospital, Heidelberg, Germany.; German Center for Infection Research, Heidelberg, Germany., Bosinger S; Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA., Stanic M; Heidelberg University Hospital, Heidelberg, Germany., Gregoretti F; Institute for High Performance Computing and Networking, ICAR-CNR, Naples, Italy., Antonelli L; Institute for High Performance Computing and Networking, ICAR-CNR, Naples, Italy., Oliva G; Institute for High Performance Computing and Networking, ICAR-CNR, Naples, Italy., Frese CK; CECAD Research Center, University of Cologne, Cologne, Germany., Trifunovic A; CECAD Research Center, University of Cologne, Cologne, Germany., Galy B; Division of Virus-Associated Carcinogenesis, German Cancer Research Centre, Heidelberg, Germany., Eibl C; Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany.; Institute of Biology, Cellular Biophysics, Humboldt Universität zu Berlin, Berlin, Germany., Silvestri G; Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA., Bicciato S; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy., Savarino A; Italian Institute of Health, Rome, Italy., Lusic M; Heidelberg University Hospital, Heidelberg, Germany.; German Center for Infection Research, Heidelberg, Germany.
المصدر: The EMBO journal [EMBO J] 2020 May 04; Vol. 39 (9), pp. e102209. Date of Electronic Publication: 2020 Mar 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
مواضيع طبية MeSH: Gene Regulatory Networks*, HIV Infections/*virology , HIV-1/*physiology , Iron/*metabolism , Promyelocytic Leukemia Protein/*metabolism, Animals ; Cell Line ; Disease Models, Animal ; HIV Infections/genetics ; HIV Infections/metabolism ; Humans ; Macaca ; Oxidation-Reduction ; Proteolysis ; Sequence Analysis, RNA ; Sumoylation ; Up-Regulation ; Virus Latency
مستخلص: HIV-1 persists in a latent form during antiretroviral therapy, mainly in CD4 + T cells, thus hampering efforts for a cure. HIV-1 infection is accompanied by metabolic alterations, such as oxidative stress, but the effect of cellular antioxidant responses on viral replication and latency is unknown. Here, we show that cells survive retroviral replication, both in vitro and in vivo in SIVmac-infected macaques, by upregulating antioxidant pathways and the intertwined iron import pathway. These changes are associated with remodeling of promyelocytic leukemia protein nuclear bodies (PML NBs), an important constituent of nuclear architecture and a marker of HIV-1 latency. We found that PML NBs are hyper-SUMOylated and that PML protein is degraded via the ubiquitin-proteasome pathway in productively infected cells, before latency establishment and after reactivation. Conversely, normal numbers of PML NBs were restored upon transition to latency or by decreasing oxidative stress or iron content. Our results highlight antioxidant and iron import pathways as determinants of HIV-1 latency and support their pharmacologic inhibition as tools to regulate PML stability and impair latency establishment.
(© 2020 The Authors. Published under the terms of the CC BY 4.0 license.)
References: Nat Commun. 2018 Apr 20;9(1):1581. (PMID: 29679077)
Viruses. 2015 Dec 23;8(1):. (PMID: 26703718)
Mol Ther Nucleic Acids. 2012 Sep 18;1:e43. (PMID: 23344235)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Cell Cycle. 2014;13(9):1400-12. (PMID: 24626186)
Front Biosci (Landmark Ed). 2009 Jan 01;14(4):1325-36. (PMID: 19273133)
J Biol Chem. 2015 Jul 24;290(30):18361-9. (PMID: 26063799)
J Cell Biol. 2012 Nov 12;199(4):583-8. (PMID: 23148232)
Retrovirology. 2010 Dec 02;7:104. (PMID: 21126372)
J Biol Chem. 2015 Jan 9;290(2):1020-38. (PMID: 25406321)
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8869-73. (PMID: 9671771)
Cell Host Microbe. 2013 Jun 12;13(6):665-77. (PMID: 23768491)
PLoS Pathog. 2015 Jun 11;11(6):e1004955. (PMID: 26067822)
Adv Sci (Weinh). 2019 May 07;6(13):1900319. (PMID: 31380187)
Chem Biol Interact. 1994 Jun;91(2-3):79-89. (PMID: 8194137)
Cancer Lett. 2015 Jan 1;356(1):58-71. (PMID: 24370566)
Curr Opin Genet Dev. 2019 Apr;55:100-105. (PMID: 31479982)
Mol Metab. 2017 Jul 12;6(9):1024-1039. (PMID: 28951826)
Retrovirology. 2015 May 16;12:42. (PMID: 25980942)
Nat Rev Drug Discov. 2013 Dec;12(12):931-47. (PMID: 24287781)
J Virol. 2015 Aug;89(15):7521-35. (PMID: 25972547)
AIDS Res Hum Retroviruses. 2016 Feb;32(2):187-93. (PMID: 26171776)
Autophagy. 2018;14(10):1693-1709. (PMID: 29938581)
Cell Rep. 2017 Oct 17;21(3):813-822. (PMID: 29045846)
J Virol. 2006 Feb;80(4):1939-48. (PMID: 16439549)
Free Radic Biol Med. 2009 Aug 1;47(3):261-8. (PMID: 19409485)
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. (PMID: 20057044)
Mol Biol Cell. 2014 Aug 15;25(16):2485-98. (PMID: 24943846)
Cytokine. 2007 Jul;39(1):63-74. (PMID: 17643313)
Annu Rev Pharmacol Toxicol. 2010;50:323-54. (PMID: 20055707)
PLoS Pathog. 2016 Apr 19;12(4):e1005581. (PMID: 27093399)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Bioinformatics. 2011 Jun 15;27(12):1739-40. (PMID: 21546393)
J Exp Med. 2017 Nov 6;214(11):3197-3206. (PMID: 28931625)
Cell. 2017 Jan 26;168(3):344-361. (PMID: 28129536)
Virology. 2007 Oct 25;367(2):324-33. (PMID: 17631934)
J Clin Virol. 2001 Feb;20(3):141-7. (PMID: 11166663)
EMBO J. 2003 Apr 15;22(8):1868-77. (PMID: 12682019)
Am J Pathol. 1979 Jun;95(3):663-73. (PMID: 377994)
J Clin Invest. 2016 May 2;126(5):1630-9. (PMID: 27135880)
Mol Cell. 2001 Jun;7(6):1245-54. (PMID: 11430827)
Antioxid Redox Signal. 2018 Dec 10;29(17):1756-1773. (PMID: 28793787)
Cold Spring Harb Perspect Med. 2011 Sep;1(1):a007096. (PMID: 22229121)
IEEE Trans Image Process. 1993;2(2):176-201. (PMID: 18296207)
Annu Rev Pharmacol Toxicol. 2013;53:401-26. (PMID: 23294312)
J Clin Invest. 2014 Oct;124(10):4459-72. (PMID: 25202977)
Nat Med. 2009 Aug;15(8):893-900. (PMID: 19543283)
J Cell Biol. 2014 Mar 17;204(6):931-45. (PMID: 24637324)
Cell Biochem Funct. 1999 Mar;17(1):47-55. (PMID: 10191508)
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002)
Blood. 2009 Jan 1;113(1):58-65. (PMID: 18849485)
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. (PMID: 16199517)
Nat Rev Microbiol. 2008 Jul;6(7):541-52. (PMID: 18552864)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
PLoS Pathog. 2015 Nov 13;11(11):e1005280. (PMID: 26566030)
Curr Opin HIV AIDS. 2013 Jul;8(4):255-61. (PMID: 23615116)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
J Biol Chem. 2014 Oct 10;289(41):28121-8. (PMID: 25160623)
Nat Protoc. 2013 Sep;8(9):1660-9. (PMID: 23928499)
Science. 2010 Apr 9;328(5975):240-3. (PMID: 20378816)
EMBO J. 2020 May 4;39(9):e102209. (PMID: 32157726)
Cancer Res. 2006 Jun 15;66(12):6192-8. (PMID: 16778193)
Int J Antimicrob Agents. 2019 Nov;54(5):592-600. (PMID: 31394172)
Curr Opin Cell Biol. 2018 Jun;52:154-161. (PMID: 29723661)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
BMC Res Notes. 2010 Nov 10;3:294. (PMID: 21067583)
Nat Med. 2018 Feb;24(2):186-193. (PMID: 29334375)
Retrovirology. 2013 Jul 16;10:71. (PMID: 23866829)
Cell Metab. 2019 Mar 5;29(3):611-626.e5. (PMID: 30581119)
J Clin Virol. 2004 Dec;31 Suppl 1:S92-8. (PMID: 15567100)
Genome Res. 2019 Jun;29(6):883-895. (PMID: 31097473)
AIDS Res Hum Retroviruses. 2015 Mar;31(3):305-12. (PMID: 25291189)
Cell Death Dis. 2013 Dec 05;4:e944. (PMID: 24309931)
J Clin Invest. 2015 Dec;125(12):4497-513. (PMID: 26551680)
Nature. 1999 Oct 28;401(6756):874-5. (PMID: 10553903)
Free Radic Biol Med. 1995 Oct;19(4):523-8. (PMID: 7590404)
J Biol Chem. 1993 Feb 5;268(4):2917-23. (PMID: 8428966)
Nucleus. 2014;5(6):499-507. (PMID: 25482067)
Nat Struct Mol Biol. 2013 Apr;20(4):525-31. (PMID: 23503365)
Nat Protoc. 2019 Jan;14(1):68-85. (PMID: 30464214)
معلومات مُعتمدة: 04.704 International Deutsches Zentrum für Infektionsforschung (DZIF); 04.810 International Deutsches Zentrum für Infektionsforschung (DZIF); International Gilead Sciences (Gilead); International Humboldt Foundation
فهرسة مساهمة: Keywords: HIV-1 latency; iron; oxidative stress; promyelocytic leukemia protein; proteasome
سلسلة جزيئية: GEO GSE127468; GSE73232
PDB 5yuf
المشرفين على المادة: 0 (Promyelocytic Leukemia Protein)
143220-95-5 (PML protein, human)
E1UOL152H7 (Iron)
تواريخ الأحداث: Date Created: 20200312 Date Completed: 20201211 Latest Revision: 20231113
رمز التحديث: 20231113
مُعرف محوري في PubMed: PMC7196916
DOI: 10.15252/embj.2019102209
PMID: 32157726
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2075
DOI:10.15252/embj.2019102209