دورية أكاديمية
Ectopic expression of IiSHP2 from Isatis indigotica Fortune, a PLE-lineage MADS-box gene, influences leaf, floral organ and silique morphology in Arabidopsis thaliana .
| العنوان: | Ectopic expression of IiSHP2 from Isatis indigotica Fortune, a PLE-lineage MADS-box gene, influences leaf, floral organ and silique morphology in Arabidopsis thaliana . |
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| المؤلفون: | Lu MX; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China., Li DZ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China., Pu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China., Ma YQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China., Huang X; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China., Xu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069 China. |
| المصدر: | Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2020 Feb; Vol. 26 (2), pp. 379-389. Date of Electronic Publication: 2020 Jan 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Prof. H.S. Srivastava Foundation for Science and Technology Country of Publication: India NLM ID: 101304333 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0971-5894 (Print) Linking ISSN: 09740430 NLM ISO Abbreviation: Physiol Mol Biol Plants Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2008- : New Delhi : Springer India Private Ltd., : Prof. H.S. Srivastava Foundation for Science and Technology Original Publication: Lucknow : Prof. H.S. Srivastava Foundation for Science and Technology |
| مستخلص: | In order to ascertain the regulatory mechanism of fruit development in Isatis indigotica Fortune, the complementary DNA (cDNA) sequence of the SHATTERPROOF 2 ( SHP2 ) orthologous gene was identified by Rapid Amplification of cDNA Ends technology and the corresponding gene was named IiSHP2 . The expression pattern of IiSHP2 was determined by quantitative reverse transcription-polymerase chain reaction and wild-type Col-0 Arabidopsis plants were transformed with the IiSHP2 gene using Agrobacterium tumefaciens and the floral-dip method. Expression analyses indicated that IiSHP2 was highly expressed in flowers, silicles and seeds. Compared to wild-type plants, IiSHP2 transgenic lines bolted earlier. Detailed phenotypic observations showed that the size of the rosette and cauline leaves in transgenic lines was reduced and the cauline leaves of the transgenic lines were incurved and displayed a funnel-like shape. During the reproductive growth stage, IiSHP2 transgenic plants produced shortened sepals and the flower buds were not encapsulated completely. Moreover, the petals of the transgenic lines were converted into stamineous tissues, accompanied by exposed stamens, short malformed siliques and wrinkled valves, indicating a severe decline in fertility. These experimental conclusions are valuable as a reference for the breeding of medicinal plants. Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest. (© Prof. H.S. Srivastava Foundation for Science and Society 2020.) |
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| فهرسة مساهمة: | Keywords: Floral differentiation; IiSHP2; Isatis indigotica Fortune; MADS-box gene; Silique development |
| تواريخ الأحداث: | Date Created: 20200312 Latest Revision: 20210201 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7036382 |
| DOI: | 10.1007/s12298-019-00745-6 |
| PMID: | 32158142 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0971-5894 |
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| DOI: | 10.1007/s12298-019-00745-6 |