دورية أكاديمية
[Design of Guide RNA for CRISPR/Cas Plant Genome Editing].
| العنوان: | [Design of Guide RNA for CRISPR/Cas Plant Genome Editing]. |
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| المؤلفون: | Gerashchenkov GA; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia.; apomixis@anrb.ru., Rozhnova NA; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Kuluev BR; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Kiryanova OY; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia.; Ufa State Petroleum Technological University, Ufa, 450062 Russia., Gumerova GR; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Knyazev AV; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Vershinina ZR; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Mikhailova EV; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Chemeris DA; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Matniyazov RT; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Baimiev AK; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Gubaidullin IM; Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, Ufa, 450075 Russia., Baimiev AK; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia., Chemeris AV; Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa, 450054 Russia. |
| المصدر: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2020 Jan-Feb; Vol. 54 (1), pp. 29-50. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | CRISPR-Cas Systems/*genetics , Gene Editing/*methods , Genome, Plant/*genetics , RNA, Guide, CRISPR-Cas Systems/*genetics |
| مستخلص: | CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide sequence (protospacer) in the gene to be edited is important in the design of guide RNA, which can be carried out by specialized software. We review and compare all the known on-line and off-line resources for guide RNA design, with special attention paid to tools capable of searching for off-target edits sites in plant genomes. The use of Cas12a may be preferable to Cas9. Techniques allowing C→T and G→A base editing without DNA cleavage are discussed along with the basic requirements for the design of effective and highly specific guide RNAs. Ways for improving guide RNA design software are presented. We also discuss the lesser risks of off-target editing in plant genomes as opposed to animal genomes. Examples of edited plant genomes including those that do not lead to the creation of transgenic plants are reviewed. |
| فهرسة مساهمة: | Keywords: CRISPR; Cas12a; Cas9; Cpf1; PAM; computer program; guide RNA; guide sequence; off-target; protospacer; spacer; target site; web-resource |
| المشرفين على المادة: | 0 (RNA, Guide, CRISPR-Cas Systems) |
| تواريخ الأحداث: | Date Created: 20200313 Date Completed: 20200406 Latest Revision: 20240104 |
| رمز التحديث: | 20240104 |
| DOI: | 10.31857/S0026898420010061 |
| PMID: | 32163387 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0026-8984 |
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| DOI: | 10.31857/S0026898420010061 |