التفاصيل البيبلوغرافية
| العنوان: |
A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants. |
| المؤلفون: |
Maurastoni M; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Sá-Antunes TF; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Oliveira SA; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Santos AMC; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Ventura JA; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Vitória, Epírito Santo, Brazil., Fernandes PMB; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil. patricia.fernandes@ufes.br. |
| المصدر: |
Archives of virology [Arch Virol] 2020 May; Vol. 165 (5), pp. 1211-1214. Date of Electronic Publication: 2020 Mar 13. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Wien, New York, Springer-Verlag. |
| مواضيع طبية MeSH: |
Carica/*virology , Multiplex Polymerase Chain Reaction/*methods , Plant Diseases/*virology , Reverse Transcriptase Polymerase Chain Reaction/*methods , Totiviridae/*isolation & purification, Molecular Diagnostic Techniques/methods ; Totiviridae/classification ; Totiviridae/genetics |
| مستخلص: |
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction. |
| معلومات مُعتمدة: |
304719/2014-5 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 308631/2016-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| تواريخ الأحداث: |
Date Created: 20200315 Date Completed: 20200421 Latest Revision: 20200421 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1007/s00705-020-04588-5 |
| PMID: |
32170392 |
| قاعدة البيانات: |
MEDLINE |
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