دورية أكاديمية
أعرض في EDS

Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients.

التفاصيل البيبلوغرافية
العنوان: Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients.
المؤلفون: Gryp T; Department of Internal Medicine and Pediatrics, Nephrology Section, Ghent University Hospital, 9000 Ghent, Belgium.; Department of Diagnostic Sciences, Laboratory Bacteriology Research, Ghent University, 9000 Ghent, Belgium.; Department of Microbiology, Immunology and Transplantation, Molecular Microbiology-Microbiome Research Lab, KU Leuven, 3000 Leuven, Belgium., Huys GRB; Department of Microbiology, Immunology and Transplantation, Molecular Microbiology-Microbiome Research Lab, KU Leuven, 3000 Leuven, Belgium., Joossens M; Department of Microbiology, Immunology and Transplantation, Molecular Microbiology-Microbiome Research Lab, KU Leuven, 3000 Leuven, Belgium., Van Biesen W; Department of Internal Medicine and Pediatrics, Nephrology Section, Ghent University Hospital, 9000 Ghent, Belgium., Glorieux G; Department of Internal Medicine and Pediatrics, Nephrology Section, Ghent University Hospital, 9000 Ghent, Belgium., Vaneechoutte M; Department of Diagnostic Sciences, Laboratory Bacteriology Research, Ghent University, 9000 Ghent, Belgium.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2020 Mar 14; Vol. 21 (6). Date of Electronic Publication: 2020 Mar 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Bacteria/*metabolism , Cresols/*metabolism , Indican/*metabolism , Indoleacetic Acids/*metabolism , Renal Insufficiency, Chronic/*pathology , Sulfuric Acid Esters/*metabolism, Amino Acids, Aromatic/metabolism ; Bacteria/classification ; Bacteria/isolation & purification ; Feces/microbiology ; Gastrointestinal Microbiome/physiology ; Humans ; Toxins, Biological/metabolism
مستخلص: In chronic kidney disease (CKD), impaired kidney function results in accumulation of uremic toxins, which exert deleterious biological effects and contribute to inflammation and cardiovascular morbidity and mortality. Protein-bound uremic toxins (PBUTs), such as p -cresyl sulfate, indoxyl sulfate and indole-3-acetic acid, originate from phenolic and indolic compounds, which are end products of gut bacterial metabolization of aromatic amino acids (AAA). This study investigates gut microbial composition at different CKD stages by isolating, identifying and quantifying PBUT precursor-generating bacteria. Fecal DNA extracts from 14 controls and 138 CKD patients were used to quantify total bacterial number and 11 bacterial taxa with qPCR. Moreover, isolated bacteria from CKD 1 and CKD 5 fecal samples were cultured in broth medium supplemented with AAA under aerobic and anaerobic conditions, and classified as PBUT precursor-generators based on their generation capacity of phenolic and indolic compounds, measured with U(H)PLC. In total, 148 different fecal bacterial species were isolated, of which 92 were PBUT precursor-generators. These bacterial species can be a potential target for reducing PBUT plasma levels in CKD. qPCR indicated lower abundance of short chain fatty acid-generating bacteria, Bifidobacterium spp. and Streptococcus spp., and higher Enterobacteriaceae and E. coli with impaired kidney function, confirming an altered gut microbial composition in CKD.
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معلومات مُعتمدة: G017815N Fonds Wetenschappelijk Onderzoek
فهرسة مساهمة: Keywords: aromatic amino acids; bacterial culture; bacterial metabolization; chronic kidney disease; fecal bacteria; indole; indole-3-acetic acid; p-cresol; phenol; protein-bound uremic toxins; qPCR
المشرفين على المادة: 0 (Amino Acids, Aromatic)
0 (Cresols)
0 (Indoleacetic Acids)
0 (Sulfuric Acid Esters)
0 (Toxins, Biological)
56M34ZQY1S (4-cresol sulfate)
6U1S09C61L (indoleacetic acid)
N187WK1Y1J (Indican)
تواريخ الأحداث: Date Created: 20200319 Date Completed: 20201204 Latest Revision: 20240328
رمز التحديث: 20240329
مُعرف محوري في PubMed: PMC7139965
DOI: 10.3390/ijms21061986
PMID: 32183306
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms21061986