دورية أكاديمية
أعرض في EDS

Addition of insoluble fiber to isolation media allows for increased metabolite diversity of lab-cultivable microbes derived from zebrafish gut samples.

التفاصيل البيبلوغرافية
العنوان: Addition of insoluble fiber to isolation media allows for increased metabolite diversity of lab-cultivable microbes derived from zebrafish gut samples.
المؤلفون: Condren AR; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA., Costa MS; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA.; Faculty of Pharmaceutical Sciences, University of Iceland , Reykjavik, Iceland., Sanchez NR; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA., Konkapaka S; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA., Gallik KL; Department of Biological Sciences, University of Illinois at Chicago , Chicago, IL, USA., Saxena A; Department of Biological Sciences, University of Illinois at Chicago , Chicago, IL, USA., Murphy BT; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA., Sanchez LM; Department of Pharmaceutical Sciences, University of Illinois at Chicago , Chicago, IL, USA.
المصدر: Gut microbes [Gut Microbes] 2020 Jul 03; Vol. 11 (4), pp. 1064-1076. Date of Electronic Publication: 2020 Mar 22.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101495343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1949-0984 (Electronic) Linking ISSN: 19490976 NLM ISO Abbreviation: Gut Microbes Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin, Tex. : Landes Bioscience
مواضيع طبية MeSH: Dietary Fiber* , Gastrointestinal Microbiome*, Bacteria/*growth & development , Intestines/*microbiology , Zebrafish/*microbiology, Animals ; Bacteria/classification ; Bacteria/isolation & purification ; Bacteria/metabolism ; Culture Media ; Phylogeny ; Secondary Metabolism
مستخلص: There is a gap in measured microbial diversity when comparing genomic sequencing techniques versus cultivation from environmental samples in a laboratory setting. Standardized methods in artificial environments may not recapitulate the environmental conditions that native microbes require for optimal growth. For example, the intestinal tract houses microbes at various pH values as well as minimal oxygen and light environments. These microbes are also exposed to an atypical source of carbon: dietary fiber compacted in fecal matter. To investigate how the addition of insoluble fiber to isolation media could affect the cultivation of microbes from zebrafish intestines, an isolate library was built and analyzed using the bioinformatics pipeline IDBac. While all isolation media encouraged the growth of species from several phyla, the extent of growth was greater with the addition of fiber allowing for easier isolation. Furthermore, fiber addition altered the metabolism of the cultivated gut-derived microbes and induced the production of unique metabolites that were not produced when microbes were otherwise grown on standard isolation media. Addition of this inexpensive carbon source to the media supported the cultivation of a diverse community whose secondary metabolite production may more closely replicate their metabolite production in vivo .
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معلومات مُعتمدة: R01 GM125943 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: in vitro cultivation; Gut microbes; insoluble fiber; metabolomics; natural products; zebrafish
المشرفين على المادة: 0 (Culture Media)
0 (Dietary Fiber)
تواريخ الأحداث: Date Created: 20200324 Date Completed: 20210702 Latest Revision: 20210702
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC7524352
DOI: 10.1080/19490976.2020.1740073
PMID: 32202200
قاعدة البيانات: MEDLINE
الوصف
تدمد:1949-0984
DOI:10.1080/19490976.2020.1740073