دورية أكاديمية
أعرض في EDS

Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach.

التفاصيل البيبلوغرافية
العنوان: Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach.
المؤلفون: Scheideler OJ; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA., Yang C; Department of Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA., Kozminsky M; California Institute for Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, CA 94720, USA., Mosher KI; California Institute for Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, CA 94720, USA., Falcón-Banchs R; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA., Ciminelli EC; Department of Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA., Bremer AW; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA., Chern SA; Department of Physics, Harvard University, 17 Oxford Street, Cambridge, MA 02138, USA., Schaffer DV; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA.; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, 201 Gilman Hall, Berkeley, CA 94720, USA.; Helen Wills Neuroscience Institute, University of California, Berkeley, 132 Barker Hall #3190, Berkeley, CA 94720, USA., Sohn LL; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA 94720, USA.; Department of Mechanical Engineering, University of California, Berkeley, 5118 Etcheverry Hall, Berkeley, CA 94720, USA.
المصدر: Science advances [Sci Adv] 2020 Mar 18; Vol. 6 (12), pp. eaay5696. Date of Electronic Publication: 2020 Mar 18 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: eCollection Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
مواضيع طبية MeSH: DNA* , Models, Biological* , Signal Transduction*, Neurons/*physiology, Animals ; Biomarkers ; Cells, Cultured ; Humans ; Ligands ; Rats
مستخلص: Elucidating how the spatial organization of extrinsic signals modulates cell behavior and drives biological processes remains largely unexplored because of challenges in controlling spatial patterning of multiple microenvironmental cues in vitro. Here, we describe a high-throughput method that directs simultaneous assembly of multiple cell types and solid-phase ligands across length scales within minutes. Our method involves lithographically defining hierarchical patterns of unique DNA oligonucleotides to which complementary strands, attached to cells and ligands-of-interest, hybridize. Highlighting our method's power, we investigated how the spatial presentation of self-renewal ligand fibroblast growth factor-2 (FGF-2) and differentiation signal ephrin-B2 instruct single adult neural stem cell (NSC) fate. We found that NSCs have a strong spatial bias toward FGF-2 and identified an unexpected subpopulation exhibiting high neuronal differentiation despite spatially occupying patterned FGF-2 regions. Overall, our broadly applicable, DNA-directed approach enables mechanistic insight into how tissues encode regulatory information through the spatial presentation of heterogeneous signals.
(Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
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معلومات مُعتمدة: F32 CA243354 United States CA NCI NIH HHS; R01 CA190843 United States CA NCI NIH HHS; R21 CA182375 United States CA NCI NIH HHS; R21 EB019181 United States EB NIBIB NIH HHS
المشرفين على المادة: 0 (Biomarkers)
0 (Ligands)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20200325 Date Completed: 20201124 Latest Revision: 20210117
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7080440
DOI: 10.1126/sciadv.aay5696
PMID: 32206713
قاعدة البيانات: MEDLINE
الوصف
تدمد:2375-2548
DOI:10.1126/sciadv.aay5696