دورية أكاديمية
Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors.
| العنوان: | Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors. |
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| المؤلفون: | Castiglioni GZ; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil., Bettio G; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil., Matte CR; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil., Jacques RA; Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil., Dos Santos Polidoro A; Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil., Rosa CA; Department of Microbiology, ICB, C.P. 486, State University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil., Ayub MAZ; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br. |
| المصدر: | Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2020 Aug; Vol. 43 (8), pp. 1391-1402. Date of Electronic Publication: 2020 Mar 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, Germany : Springer-Verlag, 2001- |
| مواضيع طبية MeSH: | Enzymes, Immobilized/*chemistry , Geotrichum/*growth & development , Lipase/*chemistry , Plant Oils/*metabolism , Seeds/*chemistry , Vitis/*chemistry , Volatile Organic Compounds/*metabolism, Hydrolysis ; Plant Oils/chemistry |
| مستخلص: | Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L -1 min -1 in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L -1 min -1 , using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min -1 at 45 °C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC-MS as γ-octalactone and γ-nonalactone. |
| معلومات مُعتمدة: | Financing Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| فهرسة مساهمة: | Keywords: Enzymatic hydrolysis; Galactomyces geotrichum; Geotrichum klebahnii; Grape seed oil; Lactone bioproduction; Packed-bed reactor |
| المشرفين على المادة: | 0 (Enzymes, Immobilized) 0 (Plant Oils) 0 (Volatile Organic Compounds) EC 3.1.1.3 (Lipase) |
| SCR Organism: | Geotrichum candidum; Geotrichum klebahnii |
| تواريخ الأحداث: | Date Created: 20200325 Date Completed: 20210609 Latest Revision: 20210609 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00449-020-02334-4 |
| PMID: | 32206907 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1615-7605 |
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| DOI: | 10.1007/s00449-020-02334-4 |