دورية أكاديمية
[Expression of the NMDA receptor in the rat model of orchialgia].
| العنوان: | [Expression of the NMDA receptor in the rat model of orchialgia]. |
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| المؤلفون: | Tian K; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Yu JW; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Zhuang JT; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Zhou MK; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Feng X; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Long SY; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Wang FL; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Zhou WL; School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, China., Deng CH; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Tu XA; Department of Urology and Andrology, The First Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China. |
| المصدر: | Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2019 Apr; Vol. 25 (5), pp. 296-301. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Nanjing Jun Qu Nanjing Zong Yi Yuan zhu ban, Zhonghua Nan Ke Xue Za Zhi Bian Ji Bu bian ji chu ban Country of Publication: China NLM ID: 101093592 Publication Model: Print Cited Medium: Internet ISSN: 1009-3591 (Print) Linking ISSN: 10093591 NLM ISO Abbreviation: Zhonghua Nan Ke Xue Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Nanjing : Nanjing Jun Qu Nanjing Zong Yi Yuan zhu ban, Zhonghua Nan Ke Xue Za Zhi Bian Ji Bu bian ji chu ban, |
| مواضيع طبية MeSH: | Receptors, N-Methyl-D-Aspartate/*metabolism , Testicular Diseases/*metabolism, Animals ; Ganglia, Spinal/metabolism ; Hyperalgesia ; Male ; Pain ; Rats ; Rats, Sprague-Dawley ; Spinal Cord Dorsal Horn/metabolism |
| مستخلص: | Objective: To explore the expression of the N-methyl-D-aspartate (NMDA) receptor in the rat model of orchialgia and its possible mechanisms. Methods: According to Yoshioka's method, the male rats in the control group were injected with 0.2 ml saline, and those in the experimental group with 0.2 ml 2% acetic acid solution. Then we tested the behavioral responses of the rats and determined the expressions of the subunits NR1 and NR2B of the NMDA receptor in the dorsal root ganglion and spinal dorsal horn by Western blot, RT-qPCR and immunofluorescence staining. Results: The withdrawal latency was decreased in the model rats, reaching the lowest value at 4 hours after modeling, significantly lower than in the controls (［4.15 ± 0.84］ vs ［12.32 ± 1.05］, P < 0.05). Compared with the controls, the model rats showed remarkably increased mRNA and protein expressions of NR2B in the dorsal root ganglion (P < 0.05) but not in the spinal dorsal horn at 4 hours. However, no statistically significant difference was found in the expression of NR1 either in the dorsal root ganglion or in the spinal dorsal horn between the two groups (P > 0.05). Conclusions: The NMDA receptor plays an important role in pathogenesis of orchialgia in rats. In the early stage of pain, upregulating the expression of the subunit NR2B of the NMDA receptor can mediate peripheral hyperalgesia and consequently orchialgia. |
| فهرسة مساهمة: | Keywords: N-methyl-D-aspartate receptor; dorsal root ganglion; rat; spinal dorsal horn; orchialgia |
| المشرفين على المادة: | 0 (NR1 NMDA receptor) 0 (NR2B NMDA receptor) 0 (Receptors, N-Methyl-D-Aspartate) |
| تواريخ الأحداث: | Date Created: 20200328 Date Completed: 20200420 Latest Revision: 20220413 |
| رمز التحديث: | 20231215 |
| PMID: | 32216209 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-3591 |
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