دورية أكاديمية
DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness.
| العنوان: | DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness. |
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| المؤلفون: | Bush A; Environment and Climate Change Canada, Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada; alexalbush@gmail.com.; Lancaster Environment Centre, University of Lancaster, Lancaster LA1 4YW, United Kingdom., Monk WA; Environment and Climate Change Canada, Canadian Rivers Institute, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB E3B 5A3, Canada., Compson ZG; Environment and Climate Change Canada, Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.; Centre for Environmental Genomics Applications, St. John's, NL A1A 0R6, Canada., Peters DL; Watershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, University of Victoria, Victoria, BC V8P 5C2, Canada., Porter TM; Great Lakes Forestry Centre, Natural Resources Canada, Sault Ste. Marie, ON P6A 2E5, Canada.; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON N1G 2W1, Canada.; Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Shokralla S; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON N1G 2W1, Canada.; Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Wright MTG; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON N1G 2W1, Canada.; Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Hajibabaei M; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON N1G 2W1, Canada.; Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Baird DJ; Environment and Climate Change Canada, Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Apr 14; Vol. 117 (15), pp. 8539-8545. Date of Electronic Publication: 2020 Mar 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Biodiversity* , Ecosystem* , Wetlands*, DNA/*analysis , DNA Barcoding, Taxonomic/*methods , Environmental Monitoring/*methods , Invertebrates/*physiology, Animals ; Wilderness |
| مستخلص: | The complexity and natural variability of ecosystems present a challenge for reliable detection of change due to anthropogenic influences. This issue is exacerbated by necessary trade-offs that reduce the quality and resolution of survey data for assessments at large scales. The Peace-Athabasca Delta (PAD) is a large inland wetland complex in northern Alberta, Canada. Despite its geographic isolation, the PAD is threatened by encroachment of oil sands mining in the Athabasca watershed and hydroelectric dams in the Peace watershed. Methods capable of reliably detecting changes in ecosystem health are needed to evaluate and manage risks. Between 2011 and 2016, aquatic macroinvertebrates were sampled across a gradient of wetland flood frequency, applying both microscope-based morphological identification and DNA metabarcoding. By using multispecies occupancy models, we demonstrate that DNA metabarcoding detected a much broader range of taxa and more taxa per sample compared to traditional morphological identification and was essential to identifying significant responses to flood and thermal regimes. We show that family-level occupancy masks high variation among genera and quantify the bias of barcoding primers on the probability of detection in a natural community. Interestingly, patterns of community assembly were nearly random, suggesting a strong role of stochasticity in the dynamics of the metacommunity. This variability seriously compromises effective monitoring at local scales but also reflects resilience to hydrological and thermal variability. Nevertheless, simulations showed the greater efficiency of metabarcoding, particularly at a finer taxonomic resolution, provided the statistical power needed to detect change at the landscape scale. Competing Interests: The authors declare no competing interest. (Copyright © 2020 the Author(s). Published by PNAS.) |
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| فهرسة مساهمة: | Keywords: detectability; occupancy; power analysis; stochasticity; taxonomic resolution |
| المشرفين على المادة: | 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20200329 Date Completed: 20200721 Latest Revision: 20200721 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7165428 |
| DOI: | 10.1073/pnas.1918741117 |
| PMID: | 32217735 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.1918741117 |