دورية أكاديمية
Valorization of two agroindustrial wastes to produce alpha-amylase enzyme from Aspergillus oryzae by solid-state fermentation.
| العنوان: | Valorization of two agroindustrial wastes to produce alpha-amylase enzyme from Aspergillus oryzae by solid-state fermentation. |
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| المؤلفون: | Melnichuk N; Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina; Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina., Braia MJ; Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina; Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina., Anselmi PA; Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina., Meini MR; Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina; Área Biofísica, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina., Romanini D; Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina; Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina. Electronic address: romanini@iprobyq-conicet.gob.ar. |
| المصدر: | Waste management (New York, N.Y.) [Waste Manag] 2020 Apr 01; Vol. 106, pp. 155-161. Date of Electronic Publication: 2020 Mar 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: United States NLM ID: 9884362 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2456 (Electronic) Linking ISSN: 0956053X NLM ISO Abbreviation: Waste Manag Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Pergamon Press, c1989- |
| مواضيع طبية MeSH: | Aspergillus oryzae* , alpha-Amylases*, Fermentation ; Temperature ; Triticum |
| مستخلص: | The global amount of soybean and wheat produced is about 350 and 750 million metric tons every year, respectively. In consequence, huge amounts of waste are produced from them. The aim of this work was to employ two wastes -soybean husk and flour mill waste- to produce high quantities of alpha-amylase enzyme. The substrate composition and the culture conditions were assayed to improve alpha-amylase production by solid-state fermentation employing the fungus Aspergillus oryzae. The maximum productivity of the enzyme was achieved using a culture substrate composed of the two wastes, at 45% soybean husk and 55% flour mill by-product, without pre-treatment, at an incubation temperature of 30 °C. The optimal incubation time (6 days), yielded a very high alpha-amylase activity (47,000 U/g dry substrate) at low-cost. The enzymatic extract obtained was characterized by LC-MS, providing a complete profile of the proteins produced during the solid-state fermentation on these two wastes. Then, the extract was purified in a single-step by size-exclusion chromatography and the recovery and the purification factor of alpha-amylase enzyme were about 83% and 6, respectively. The system was scaled up 50 times and yielded a similar enzymatic activity (45,900 U/g of dry substrate). Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Alpha-amylase; Aspergillus oryzae; Flour mill waste; Solid-state fermentation; Soybean husk |
| المشرفين على المادة: | EC 3.2.1.1 (alpha-Amylases) |
| تواريخ الأحداث: | Date Created: 20200330 Date Completed: 20200414 Latest Revision: 20200414 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.wasman.2020.03.025 |
| PMID: | 32220823 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-2456 |
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| DOI: | 10.1016/j.wasman.2020.03.025 |