دورية أكاديمية
Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs.
| العنوان: | Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs. |
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| المؤلفون: | Ehsan M; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, People's Republic of China.; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Gadahi JA; Department of Veterinary Parasitology, Sindh Agriculture University Tandojam, Hyderabad, Pakistan., Liu T; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Lu M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Wang Y; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Hasan MW; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Haseeb M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Yan R; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Xu L; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Song X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China., Zhu XQ; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, People's Republic of China., Li X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China. lixiangrui@njau.edu.cn. |
| المصدر: | Parasites & vectors [Parasit Vectors] 2020 Mar 30; Vol. 13 (1), pp. 154. Date of Electronic Publication: 2020 Mar 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central |
| مواضيع طبية MeSH: | Goats/*parasitology , Haemonchus/*enzymology , Haemonchus/*genetics , Leukocytes, Mononuclear/*immunology , Methyltransferases/*genetics , Methyltransferases/*immunology , Methyltransferases/*isolation & purification, Animals ; Antibodies, Helminth/blood ; Cell Proliferation ; Cloning, Molecular ; Cytokines/metabolism ; Disease Models, Animal ; Escherichia coli/genetics ; Female ; Gene Expression Regulation ; Haemonchiasis/parasitology ; Haemonchiasis/veterinary ; Helminth Proteins/genetics ; Host-Parasite Interactions/immunology ; Male ; Methyltransferases/metabolism ; Nitric Oxide/metabolism ; Rats, Sprague-Dawley ; Recombinant Proteins/genetics ; Sequence Alignment ; Sequence Analysis, Protein |
| مستخلص: | Background: Methyltransferases (MTFs) are broad range of enzymes, which are ubiquitously expressed in diverse organisms ranging from bacteria to animals. MTFs proteins have been associated with various biological/cellular processes including transcriptional regulation, subcellular protein and RNA localization, signal transduction and DNA-damage repair. However, the role of MTFs in immune mechanism during host-parasite interaction has not been addressed yet. Results: An open reading frame (764 bp) of methyltransferase-type 12 gene of H. contortus denoted as HcMTF-12, was successfully cloned using reverse transcriptase-polymerase chain reaction (RT-PCR) followed by prokaryotic expression in Escherichia coli BL21 (DE3 strain). The recombinant HcMTF-12 protein (rHcMTF-12) was about 47 kDa along with a fusion vector protein of 18 kDa. Immunoblot results identified the native protein MTF-12 with antibodies produced in rats against rHcMT-12, whereas rHcMTF-12 protein was recognized with sera of goat experimentally infected with H. contortus. Immunohistochemical analysis revealed that the native MTF-12 protein was mainly located in the periphery (cuticle) of parasite sections as well as within the pharynx and intestinal region. An immunofluorescence assay validated that rHcMTF-12 attached to the surface of goat PBMCs. Furthermore, the cytokines transcription of IL-2, IFN-γ and IL-4 transcripts of PBMCs incubated with rHcMTF-12 were enhanced in a dose-dependent manner. The secretion of TGF-β1 and IL-10 was significantly decreased. However, IL-6 production was not significantly different as compared to the control groups. Moreover, the migration activity and nitric oxide (NO) production by PBMCs were induced considerably, whereas the proliferation of PBMCs cells was negatively affected when incubated with the rHcMTF-12 protein. Conclusions: Our findings suggest that HcMTF-12 significantly mediated the functions of PBMCs, and it might be a potential candidate for therapeutic interventions against haemonchosis. |
| References: | Parasite Immunol. 1998 May;20(5):241-7. (PMID: 9651925) Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051) Parasit Vectors. 2017 Jun 26;10(1):311. (PMID: 28651566) Res Vet Sci. 2012 Apr;92(2):259-65. (PMID: 21444098) Trends Ecol Evol. 2008 Jun;23(6):318-26. (PMID: 18439709) PLoS Pathog. 2015 Jul 06;11(7):e1004994. (PMID: 26147567) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Biotechnol Adv. 2015 Dec;33(8):1744-54. (PMID: 26239368) J Mol Biol. 1995 Nov 3;253(4):618-32. (PMID: 7473738) Curr Immunol Rev. 2013;9(3):157-168. (PMID: 25383073) J Clin Psychiatry. 2017 Jun;78(6):e656-e667. (PMID: 28682528) Cell. 1995 Jul 14;82(1):143-53. (PMID: 7606780) Parasitology. 2000;120 Suppl:S63-72. (PMID: 10874710) Int J Parasitol. 2001 Dec;31(14):1563-71. (PMID: 11730782) Methods Enzymol. 1981;77:263-6. (PMID: 6276654) Microbes Infect. 1999 Dec;1(15):1297-304. (PMID: 10611758) Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):138-45. (PMID: 18187208) Int J Parasitol. 2006 Oct;36(12):1227-39. (PMID: 16889782) Arch Biochem Biophys. 1994 May 1;310(2):417-27. (PMID: 8179327) Vet Parasitol. 2012 May 4;186(1-2):70-8. (PMID: 22154968) J Am Vet Med Assoc. 2003 Aug 15;223(4):495-500. (PMID: 12930089) Vet Parasitol. 2011 Sep 27;181(2-4):239-47. (PMID: 21570191) Oncotarget. 2016 Dec 20;7(51):83926-83937. (PMID: 27893414) J Mol Biol. 1995 Mar 17;247(1):16-20. (PMID: 7897657) Epigenomics. 2009 Oct 1;1(1):163-175. (PMID: 20582239) Vet Parasitol. 2017 Aug 30;243:100-104. (PMID: 28807275) Wiad Parazytol. 2004;50(4):665-78. (PMID: 16862801) Parasit Vectors. 2014 Jul 23;7:342. (PMID: 25056558) Trends Immunol. 2011 Jun;32(6):278-86. (PMID: 21531623) J Cell Sci. 2007 Dec 15;120(Pt 24):4243-6. (PMID: 18057026) Eur J Immunol. 2000 Sep;30(9):2669-78. (PMID: 11009101) Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122) Lancet. 1984 Jul 28;2(8396):224. (PMID: 6146777) Cell. 1993 Jul 30;74(2):299-307. (PMID: 8343957) Exp Parasitol. 2016 Dec;171:57-66. (PMID: 27751769) Immunol Rev. 2014 Jul;260(1):183-205. (PMID: 24942690) J Immunol Methods. 2005 Oct 20;305(1):84-93. (PMID: 16125721) Eur J Immunol. 2002 Oct;32(10):2866-73. (PMID: 12355439) Nucleic Acids Res. 1989 Apr 11;17(7):2421-35. (PMID: 2717398) Angew Chem Int Ed Engl. 2014 Apr 7;53(15):3965-9. (PMID: 24616228) J Biol Chem. 1989 Nov 25;264(33):20131-9. (PMID: 2684970) Annu Rev Immunol. 2006;24:99-146. (PMID: 16551245) Front Immunol. 2018 Jul 16;9:1627. (PMID: 30061894) PLoS One. 2016 Jul 28;11(7):e0159796. (PMID: 27467391) Vet Parasitol. 2012 Apr 30;185(2-4):175-80. (PMID: 22153120) Parasite Immunol. 2007 Jun;29(6):319-26. (PMID: 17518950) J Biol Chem. 2006 Jul 28;281(30):21305-11. (PMID: 16704982) Acta Biochim Pol. 2006;53(2):245-56. (PMID: 16582985) Methods Enzymol. 1981;77:257-62. (PMID: 6276653) Oncotarget. 2016 Jun 14;7(24):35670-35679. (PMID: 27229536) Nature. 1994 Mar 24;368(6469):354-8. (PMID: 8127373) |
| معلومات مُعتمدة: | 2015CB150300 National key Basic Research Program of China (973 Program) (CN); Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) |
| فهرسة مساهمة: | Keywords: Goat; Haemonchus contortus; Host–parasite interactions; Methyltransferase-type 12; PBMC |
| المشرفين على المادة: | 0 (Antibodies, Helminth) 0 (Cytokines) 0 (Helminth Proteins) 0 (Recombinant Proteins) 31C4KY9ESH (Nitric Oxide) EC 2.1.1.- (Methyltransferases) |
| تواريخ الأحداث: | Date Created: 20200402 Date Completed: 20201123 Latest Revision: 20201123 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7106832 |
| DOI: | 10.1186/s13071-020-04028-y |
| PMID: | 32228657 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1756-3305 |
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| DOI: | 10.1186/s13071-020-04028-y |