دورية أكاديمية
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EDN1-AS , A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells.

التفاصيل البيبلوغرافية
العنوان: EDN1-AS , A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells.
المؤلفون: Douma LG; Department of Medicine, University of Florida, Gainesville, FL, United States.; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States., Solocinski K; Department of Medicine, University of Florida, Gainesville, FL, United States., Masten SH; Department of Medicine, University of Florida, Gainesville, FL, United States., Barral DH; Department of Medicine, University of Florida, Gainesville, FL, United States., Barilovits SJ; Department of Medicine, University of Florida, Gainesville, FL, United States., Jeffers LA; Department of Biochemistry, Cell and Developmental Biology, Emory University, Atlanta, GA, United States., Alder KD; Yale University School of Medicine, New Haven, CT, United States., Patel R; Department of Medicine, University of Florida, Gainesville, FL, United States., Wingo CS; Department of Medicine, University of Florida, Gainesville, FL, United States., Brown KD; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States., Cain BD; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States., Gumz ML; Department of Medicine, University of Florida, Gainesville, FL, United States.; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States.
المصدر: Frontiers in physiology [Front Physiol] 2020 Mar 13; Vol. 11, pp. 209. Date of Electronic Publication: 2020 Mar 13 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101549006 Publication Model: eCollection Cited Medium: Print ISSN: 1664-042X (Print) Linking ISSN: 1664042X NLM ISO Abbreviation: Front Physiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Endothelin-1 (ET-1) is a peptide hormone that functions as a vasoconstrictor in the vasculature, whereas in the collecting duct of the kidney it exerts blood pressure-lowering effects via natriuretic actions. Aberrant ET-1 signaling is associated with several pathological states including hypertension and chronic kidney disease. ET-1 expression is regulated largely through transcriptional control of the gene that encodes ET-1, EDN1 . Here we report a long, non-coding RNA (lncRNA) that appears to be antisense to the EDN1 gene, called EDN1-AS . Because EDN1-AS represents a potential novel mechanism to regulate ET-1 expression, we examined the regulation of EDN1-AS expression and action. A putative glucocorticoid receptor response (GR) element upstream of the predicted EDN1-AS transcription start site was identified using the ENCODE database and the UCSC genome browser. Two homozygous deletion clones of the element were generated using CRISPR/Cas9. This deletion resulted in a significant increase in the expression of EDN1-AS , which was associated with increased secretion of ET-1 peptide from HK-2 cells (two-fold increase in KO cells vs. CNTL, n = 7, P < 0.05). Phenotypic characterization of these CRISPR clones revealed a difference in cell growth rates. Using a standard growth assay, we determined that the KO1 clone exhibited a three-fold increase in growth over 8 days compared to control cells ( n = 4, P < 0.01) and the KO2 clone exhibited a two-fold increase ( n = 4, P < 0.01). These results support a role for EDN1-AS as a novel regulatory mechanism of ET-1 expression and cellular proliferation.
(Copyright © 2020 Douma, Solocinski, Masten, Barral, Barilovits, Jeffers, Alder, Patel, Wingo, Brown, Cain and Gumz.)
References: Life Sci. 2014 Nov 24;118(2):232-7. (PMID: 25010841)
Aging (Albany NY). 2019 Oct 20;11(20):8745-8759. (PMID: 31631065)
J Exp Med. 2020 Mar 2;217(3):. (PMID: 31816634)
J Am Heart Assoc. 2016 Jul 21;5(7):. (PMID: 27444511)
J Cardiovasc Transl Res. 2018 Dec;11(6):439-449. (PMID: 30171598)
Endocr Dev. 2011;20:116-126. (PMID: 21164265)
N Engl J Med. 2013 Aug 29;369(9):809-18. (PMID: 23984728)
Neurosci Lett. 2005 Mar 22;377(1):65-8. (PMID: 15722189)
Neurochem Res. 2020 Apr;45(4):741-751. (PMID: 31898085)
J Biol Chem. 1999 Jul 30;274(31):21544-54. (PMID: 10419459)
Future Med Chem. 2015;7(16):2221-42. (PMID: 26510815)
J Biol Chem. 2016 Oct 7;291(41):21541-21552. (PMID: 27535224)
Life Sci. 2014 Nov 24;118(2):141-8. (PMID: 24607774)
Front Physiol. 2013 Feb 19;4:22. (PMID: 23424003)
J Cardiovasc Pharmacol. 1998;31 Suppl 1:S489-91. (PMID: 9595521)
Hypertension. 2013 Jun;61(6):1142-5. (PMID: 23608655)
Kidney Int. 2014 Nov;86(5):896-904. (PMID: 24805108)
J Mol Endocrinol. 2019 Nov;63(4):R93-R102. (PMID: 31557726)
Adv Drug Deliv Rev. 2016 Sep 1;104:16-28. (PMID: 26549145)
FASEB J. 2011 Jan;25(1):16-28. (PMID: 20837776)
Hum Mol Genet. 2014 Sep 15;23(R1):R54-63. (PMID: 24838284)
Cell. 2017 Jul 27;170(3):522-533.e15. (PMID: 28753427)
Am J Physiol Renal Physiol. 2019 Nov 1;317(5):F1350-F1358. (PMID: 31545928)
Life Sci. 2014 Nov 24;118(2):255-62. (PMID: 24721511)
Cell. 2011 Apr 15;145(2):224-41. (PMID: 21496643)
Am J Physiol Renal Physiol. 2009 Jun;296(6):F1477-83. (PMID: 19279127)
Cell Oncol (Dordr). 2019 Feb;42(1):1-12. (PMID: 30361825)
Nat Rev Cardiol. 2019 Aug;16(8):491-502. (PMID: 30867577)
Am J Kidney Dis. 1995 Dec;26(6):934-41. (PMID: 7503068)
Hypertension. 2012 Jun;59(6):1151-6. (PMID: 22526258)
Nucleic Acids Res. 2019 Jan 8;47(D1):D135-D139. (PMID: 30371849)
J Endocr Soc. 2019 Jul 24;3(10):1917-1930. (PMID: 31598572)
Nephrol Dial Transplant. 2007 Dec;22(12):3487-94. (PMID: 17901069)
Mol Cell Endocrinol. 2007 Nov 15;278(1-2):36-43. (PMID: 17928134)
Physiol Rev. 2011 Jan;91(1):1-77. (PMID: 21248162)
Br J Clin Pharmacol. 2013 Oct;76(4):573-9. (PMID: 23228194)
Lancet. 2019 May 11;393(10184):1937-1947. (PMID: 30995972)
Wiley Interdiscip Rev RNA. 2018 Mar;9(2):. (PMID: 29341438)
Nat Rev Mol Cell Biol. 2018 Mar;19(3):143-157. (PMID: 29138516)
Genome Biol. 2010;11(5):R56. (PMID: 20507594)
Nephrology (Carlton). 2015 Jul;20(7):459-66. (PMID: 25753148)
Life Sci. 2014 Nov 24;118(2):195-9. (PMID: 24632479)
Science. 2000 Sep 29;289(5488):2344-7. (PMID: 11009419)
PLoS One. 2015 Apr 24;10(4):e0124633. (PMID: 25909470)
Nucleic Acids Res. 2014 Jan;42(Database issue):D764-70. (PMID: 24270787)
Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9828-33. (PMID: 24958884)
Biochemistry (Mosc). 2016 Jul;81(7):739-47. (PMID: 27449620)
معلومات مُعتمدة: P30 AG028740 United States AG NIA NIH HHS; R01 DK109570 United States DK NIDDK NIH HHS; R21 AG052861 United States AG NIA NIH HHS; T32 GM008169 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: CRISPR; circadian rhythm; kidney; long non-coding RNA; proximal tubule cells
تواريخ الأحداث: Date Created: 20200402 Latest Revision: 20210129
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7082230
DOI: 10.3389/fphys.2020.00209
PMID: 32231591
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-042X
DOI:10.3389/fphys.2020.00209