دورية أكاديمية
أعرض في EDS

CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma.

التفاصيل البيبلوغرافية
العنوان: CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma.
المؤلفون: Liu SJ; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; Department of Radiation Oncology, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Malatesta M; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Lien BV; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Saha P; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Thombare SS; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Hong SJ; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA.; Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco, CA, 94143, USA., Pedraza L; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Koontz M; Department of Ophthalmology, University of California, San Francisco, CA, 94143, USA., Seo K; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Department of Radiation Oncology, University of California, San Francisco, CA, 94143, USA., Horlbeck MA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, 94143, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA, 94143, USA.; California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA, 94143, USA.; Center for RNA Systems Biology, University of California, San Francisco, CA, 94143, USA., He D; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA.; Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco, CA, 94143, USA., Birk HS; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA., Jain M; UC Santa Cruz Genomics Institute, University of California, Santa Cruz, CA, 95064, USA., Olsen HE; UC Santa Cruz Genomics Institute, University of California, Santa Cruz, CA, 95064, USA., Akeson M; UC Santa Cruz Genomics Institute, University of California, Santa Cruz, CA, 95064, USA., Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, 94143, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA, 94143, USA.; California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA, 94143, USA.; Center for RNA Systems Biology, University of California, San Francisco, CA, 94143, USA., Monje M; Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA., Gupta N; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA., Raleigh DR; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA.; Department of Radiation Oncology, University of California, San Francisco, CA, 94143, USA., Ullian EM; Department of Ophthalmology, University of California, San Francisco, CA, 94143, USA., Lim DA; Department of Neurological Surgery, University of California, San Francisco, CA, 94143, USA. Daniel.Lim@ucsf.edu.; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, 94143, USA. Daniel.Lim@ucsf.edu.; San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA. Daniel.Lim@ucsf.edu.
المصدر: Genome biology [Genome Biol] 2020 Mar 31; Vol. 21 (1), pp. 83. Date of Electronic Publication: 2020 Mar 31.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 100960660 Publication Model: Electronic Cited Medium: Internet ISSN: 1474-760X (Electronic) Linking ISSN: 14747596 NLM ISO Abbreviation: Genome Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: London, UK : BioMed Central Ltd
Original Publication: London : Genome Biology Ltd., c2000-
مواضيع طبية MeSH: CRISPR-Cas Systems*, Brain Neoplasms/*therapy , Glioblastoma/*therapy , RNA, Long Noncoding/*antagonists & inhibitors, Adult ; Astrocytes ; Brain ; Brain Neoplasms/genetics ; Brain Neoplasms/pathology ; Brain Neoplasms/radiotherapy ; Cell Line, Tumor ; Combined Modality Therapy ; Glioblastoma/genetics ; Glioblastoma/pathology ; Glioblastoma/radiotherapy ; Humans ; Oligonucleotides, Antisense ; Organoids ; Radiation Tolerance
مستخلص: Background: Long non-coding RNAs (lncRNAs) exhibit highly cell type-specific expression and function, making this class of transcript attractive for targeted cancer therapy. However, the vast majority of lncRNAs have not been tested as potential therapeutic targets, particularly in the context of currently used cancer treatments. Malignant glioma is rapidly fatal, and ionizing radiation is part of the current standard-of-care used to slow tumor growth in both adult and pediatric patients.
Results: We use CRISPR interference (CRISPRi) to screen 5689 lncRNA loci in human glioblastoma (GBM) cells, identifying 467 hits that modify cell growth in the presence of clinically relevant doses of fractionated radiation. Thirty-three of these lncRNA hits sensitize cells to radiation, and based on their expression in adult and pediatric gliomas, nine of these hits are prioritized as lncRNA Glioma Radiation Sensitizers (lncGRS). Knockdown of lncGRS-1, a primate-conserved, nuclear-enriched lncRNA, inhibits the growth and proliferation of primary adult and pediatric glioma cells, but not the viability of normal brain cells. Using human brain organoids comprised of mature neural cell types as a three-dimensional tissue substrate to model the invasive growth of glioma, we find that antisense oligonucleotides targeting lncGRS-1 selectively decrease tumor growth and sensitize glioma cells to radiation therapy.
Conclusions: These studies identify lncGRS-1 as a glioma-specific therapeutic target and establish a generalizable approach to rapidly identify novel therapeutic targets in the vast non-coding genome to enhance radiation therapy.
References: J Neurooncol. 2018 Dec;140(3):629-638. (PMID: 30206764)
Nat Biotechnol. 2016 Dec;34(12):1279-1286. (PMID: 27798563)
Cell. 2013 Feb 28;152(5):1173-83. (PMID: 23452860)
Genes Dev. 2012 May 1;26(9):891-907. (PMID: 22549954)
Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142)
Nature. 2019 May;569(7757):503-508. (PMID: 31068700)
Curr Protoc Cell Biol. 2018 Jun;79(1):e51. (PMID: 29924488)
Acta Neuropathol. 2010 Jan;119(1):7-35. (PMID: 20012068)
J Neurooncol. 1998 Nov;40(2):171-7. (PMID: 9892099)
Nat Methods. 2018 Aug;15(8):611-616. (PMID: 30013045)
Genes Dev. 2011 Sep 15;25(18):1915-27. (PMID: 21890647)
Nat Biotechnol. 2018 Nov 05;:. (PMID: 30395134)
Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677)
Nat Rev Drug Discov. 2012 Oct;11(10):751-61. (PMID: 22935759)
Cell. 2011 Mar 4;144(5):646-74. (PMID: 21376230)
Nature. 2015 Jan 29;517(7536):583-8. (PMID: 25494202)
BMC Bioinformatics. 2013 Apr 15;14:128. (PMID: 23586463)
Science. 2014 Jan 3;343(6166):84-87. (PMID: 24336571)
Mol Cancer Ther. 2011 Oct;10(10):1949-58. (PMID: 21825006)
Science. 2017 Mar 31;355(6332):. (PMID: 28360267)
Genome Biol. 2016 Apr 14;17:67. (PMID: 27081004)
Mol Cell Biol. 2001 Jun;21(11):3820-9. (PMID: 11340173)
Nat Rev Mol Cell Biol. 2010 Mar;11(3):220-8. (PMID: 20177397)
Neuron. 2017 Jun 21;94(6):1056-1070. (PMID: 28641106)
Nat Med. 2016 Nov;22(11):1220-1228. (PMID: 27783065)
Am J Hum Genet. 2014 Feb 6;94(2):246-56. (PMID: 24507776)
Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20284-9. (PMID: 24277810)
Stem Cell Reports. 2017 Dec 12;9(6):1745-1753. (PMID: 29198827)
Nature. 2019 Apr;568(7753):511-516. (PMID: 30971826)
Nat Rev Clin Oncol. 2016 Oct;13(10):627-42. (PMID: 27245279)
Nature. 2013 Sep 19;501(7467):373-9. (PMID: 23995685)
Lancet Oncol. 2009 May;10(5):459-66. (PMID: 19269895)
Nature. 2010 Apr 15;464(7291):1071-6. (PMID: 20393566)
Cell. 2017 Feb 23;168(5):890-903.e15. (PMID: 28162770)
Lancet Oncol. 2006 Mar;7(3):241-8. (PMID: 16510333)
Cell Rep. 2019 Mar 19;26(12):3203-3211.e5. (PMID: 30893594)
Nat Methods. 2018 Aug;15(8):631-639. (PMID: 30038414)
Neuron. 2017 Aug 16;95(4):779-790.e6. (PMID: 28817799)
Cell. 2015 May 7;161(4):933-45. (PMID: 25957691)
Sci Transl Med. 2015 May 6;7(286):286ra66. (PMID: 25947161)
Genome Biol. 2010;11(10):R106. (PMID: 20979621)
JAMA. 2013 Nov 6;310(17):1842-50. (PMID: 24193082)
Nature. 2015 Feb 19;518(7539):409-12. (PMID: 25470045)
Cell. 2016 May 19;165(5):1238-1254. (PMID: 27118425)
Elife. 2016 Sep 23;5:. (PMID: 27661255)
Neuro Oncol. 2015 Aug;17(8):1051-63. (PMID: 25934816)
Annu Rev Biochem. 2012;81:145-66. (PMID: 22663078)
J Exp Med. 2016 May 30;213(6):1011-28. (PMID: 27185855)
Cell. 2013 Jul 18;154(2):442-51. (PMID: 23849981)
Cell. 2015 Dec 3;163(6):1515-26. (PMID: 26627737)
Nature. 2019 Jun;570(7762):523-527. (PMID: 31168097)
Nucleic Acids Res. 2019 Jan 8;47(D1):D766-D773. (PMID: 30357393)
Neurology. 2016 Mar 8;86(10):890-7. (PMID: 26865511)
Science. 2014 Jan 3;343(6166):80-4. (PMID: 24336569)
Nature. 2018 Aug;560(7717):243-247. (PMID: 30069053)
Cancer Res. 2001 Jul 1;61(13):4956-60. (PMID: 11431323)
Stem Cell Reports. 2017 Oct 10;9(4):1221-1233. (PMID: 28966121)
Nucleic Acids Res. 2016 Jan 29;44(2):863-77. (PMID: 26578588)
Cell. 2014 Oct 23;159(3):647-61. (PMID: 25307932)
Nat Genet. 2015 Mar;47(3):199-208. (PMID: 25599403)
Clin Cancer Res. 2019 Mar 1;25(5):1455-1461. (PMID: 30498095)
Science. 2018 Apr 20;360(6386):331-335. (PMID: 29674595)
Nature. 2017 Mar 9;543(7644):199-204. (PMID: 28241135)
Science. 2019 Jun 07;364(6444):952-955. (PMID: 31171691)
Cell. 2018 Dec 13;175(7):1972-1988.e16. (PMID: 30550791)
Genome Biol. 2017 Dec 20;18(1):234. (PMID: 29262845)
Cell. 2018 May 31;173(6):1398-1412.e22. (PMID: 29731168)
Oncogene. 2003 Sep 11;22(39):8031-41. (PMID: 12970751)
Science. 2017 Jan 6;355(6320):. (PMID: 27980086)
Cancer Cell. 2016 Apr 11;29(4):452-463. (PMID: 27070700)
Cancer Res. 2003 Apr 1;63(7):1550-4. (PMID: 12670903)
N Engl J Med. 2005 Mar 10;352(10):987-96. (PMID: 15758009)
Radiother Oncol. 2014 Apr;111(1):35-40. (PMID: 24560760)
Cell. 2013 Feb 14;152(4):909-22. (PMID: 23394947)
معلومات مُعتمدة: HG010053 United States NH NIH HHS; R21 NS101395 United States NS NINDS NIH HHS; T32 GM007618 United States GM NIGMS NIH HHS; 1R21 NS101395-01 United States NH NIH HHS; F30 NS092319-01 United States NH NIH HHS; I01 BX000252 United States BX BLRD VA; R01 HG010053 United States HG NHGRI NIH HHS; 1R01NS091544 United States NH NIH HHS; R03AG063157 United States NH NIH HHS; R01 NS091544 United States NS NINDS NIH HHS; T32 HD007470 United States HD NICHD NIH HHS; R03 AG063157 United States AG NIA NIH HHS; P30 EY002162 United States EY NEI NIH HHS
فهرسة مساهمة: Keywords: CRISPRi; Cancer therapy; Glioma; Organoids; Radiation; lncRNA
المشرفين على المادة: 0 (Oligonucleotides, Antisense)
0 (RNA, Long Noncoding)
تواريخ الأحداث: Date Created: 20200403 Date Completed: 20210223 Latest Revision: 20211009
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7110660
DOI: 10.1186/s13059-020-01995-4
PMID: 32234056
قاعدة البيانات: MEDLINE
الوصف
تدمد:1474-760X
DOI:10.1186/s13059-020-01995-4