دورية أكاديمية
أعرض في EDS

pH-Controlled Coacervate-Membrane Interactions within Liposomes.

التفاصيل البيبلوغرافية
العنوان: pH-Controlled Coacervate-Membrane Interactions within Liposomes.
المؤلفون: Last MGF; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands., Deshpande S; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.; Physical Chemistry and Soft Matter, Wageningen University and Research, Stippenweg 4, 6708 WE Wageningen, The Netherlands., Dekker C; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
المصدر: ACS nano [ACS Nano] 2020 Apr 28; Vol. 14 (4), pp. 4487-4498. Date of Electronic Publication: 2020 Apr 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington D.C. : American Chemical Society
مواضيع طبية MeSH: Artificial Cells* , Liposomes*, Hydrogen-Ion Concentration ; Hydrophobic and Hydrophilic Interactions ; Microfluidics
مستخلص: Membraneless organelles formed by liquid-liquid phase separation are dynamic structures that are employed by cells to spatiotemporally regulate their interior. Indeed, complex coacervation-based phase separation is involved in a multitude of biological tasks ranging from photosynthesis to cell division to chromatin organization, and more. Here, we use an on-chip microfluidic method to control and study the formation of membraneless organelles within liposomes, using pH as the main control parameter. We show that a transmembrane proton flux that is created by a stepwise change in the external pH can readily bring about the coacervation of encapsulated components in a controlled manner. We employ this strategy to induce and study electrostatic as well as hydrophobic interactions between the coacervate and the lipid membrane. Electrostatic interactions using charged lipids efficiently recruit coacervates to the membrane and restrict their movement along the inner leaflet. Hydrophobic interactions via cholesterol-tagged RNA molecules provide even stronger interactions, causing coacervates to wet the membrane and affect the local lipid-membrane structure, reminiscent of coacervate-membrane interactions in cells. The presented technique of pH-triggered coacervation within cell-sized liposomes may find applications in synthetic cells and in studying biologically relevant phase separation reactions in a bottom-up manner.
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فهرسة مساهمة: Keywords: coacervates; liposomes; liquid−liquid phase separation; membranes; microfluidics
المشرفين على المادة: 0 (Liposomes)
تواريخ الأحداث: Date Created: 20200403 Date Completed: 20210514 Latest Revision: 20210514
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7199211
DOI: 10.1021/acsnano.9b10167
PMID: 32239914
قاعدة البيانات: MEDLINE
الوصف
تدمد:1936-086X
DOI:10.1021/acsnano.9b10167