دورية أكاديمية
أعرض في EDS

Collagen Fibril Density Modulates Macrophage Activation and Cellular Functions during Tissue Repair.

التفاصيل البيبلوغرافية
العنوان: Collagen Fibril Density Modulates Macrophage Activation and Cellular Functions during Tissue Repair.
المؤلفون: Sapudom J; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE., Mohamed WKE; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE.; Department of Genetics and Microbiology, Autonomous University of Barcelona, 08193 Barcelona, Spain., Garcia-Sabaté A; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE., Alatoom A; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE., Karaman S; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE., Mahtani N; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE., Teo JC; Division of Engineering, New York University Abu Dhabi, Abu Dhabi 129188, UAE.; Department of Mechanical and Biomedical Engineering, New York University, New York, NY 10003, USA.
المصدر: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2020 Mar 31; Vol. 7 (2). Date of Electronic Publication: 2020 Mar 31.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101676056 Publication Model: Electronic Cited Medium: Print ISSN: 2306-5354 (Print) Linking ISSN: 23065354 NLM ISO Abbreviation: Bioengineering (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2014]-
مستخلص: Monocytes circulate in the bloodstream, extravasate into the tissue and differentiate into specific macrophage phenotypes to fulfill the immunological needs of tissues. During the tissue repair process, tissue density transits from loose to dense tissue. However, little is known on how changes in tissue density affects macrophage activation and their cellular functions. In this work, monocytic cell line THP-1 cells were embedded in three-dimensional (3D) collagen matrices with different fibril density and were then differentiated into uncommitted macrophages (M PMA ) using phorbol-12-myristate-13-acetate (PMA). M PMA macrophages were subsequently activated into pro-inflammatory macrophages (M LPS/IFNγ ) and anti-inflammatory macrophages (M IL-4/IL-13 ) using lipopolysaccharide and interferon-gamma (IFNγ), and interleukin 4 (IL-4) and IL-13, respectively. Although analysis of cell surface markers, on both gene and protein levels, was inconclusive, cytokine secretion profiles, however, demonstrated differences in macrophage phenotype. In the presence of differentiation activators, M LPS/IFNγ secreted high amounts of IL-1β and tumor necrosis factor alpha (TNFα), while M0 PMA secreted similar cytokines to M IL-4/IL-13 , but low IL-8. After removing the activators and further culture for 3 days in fresh cell culture media, the secretion of IL-6 was found in high concentrations by M IL-4/IL-13 , followed by M LPS/IFNγ and M PMA . Interestingly, the secretion of cytokines is enhanced with an increase of fibril density. Through the investigation of macrophage-associated functions during tissue repair, we demonstrated that M1 LPS/IFNγ has the potential to enhance monocyte infiltration into tissue, while M IL-4/IL-13 supported fibroblast differentiation into myofibroblasts via transforming growth factor beta 1 (TGF-β1) in dependence of fibril density, suggesting a M2a-like phenotype. Overall, our results suggest that collagen fibril density can modulate macrophage response to favor tissue functions. Understanding of immune response in such complex 3D microenvironments will contribute to the novel therapeutic strategies for improving tissue repair, as well as guidance of the design of immune-modulated materials.
Competing Interests: The authors declare no conflict of interest.
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معلومات مُعتمدة: RE266 New York University Abu Dhabi; RE267 New York University Abu Dhabi; AD266 New York University Abu Dhabi
فهرسة مساهمة: Keywords: collagen fibril density; fibroblast differentiation; immunomechanobiology; macrophage; monocyte infiltration
تواريخ الأحداث: Date Created: 20200405 Latest Revision: 20240329
رمز التحديث: 20240329
مُعرف محوري في PubMed: PMC7356036
DOI: 10.3390/bioengineering7020033
PMID: 32244521
قاعدة البيانات: MEDLINE
الوصف
تدمد:2306-5354
DOI:10.3390/bioengineering7020033