دورية أكاديمية
Immune responses induced by inactivated vaccine against Aeromonas hydrophila in pacu, Piaractus mesopotamicus.
| العنوان: | Immune responses induced by inactivated vaccine against Aeromonas hydrophila in pacu, Piaractus mesopotamicus. |
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| المؤلفون: | Vaz Farias TH; Laboratory of Aquatic Organisms Pathology (Lapoa), Aquaculture Center from UNESP (CAUNESP), Rodovia de Acesso Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil., Arijo S; Department of Microbiology, Faculty of Sciences, University of Malaga, 29071, Malaga, Spain., Medina A; Department of Microbiology, Faculty of Sciences, University of Malaga, 29071, Malaga, Spain., Pala G; Department of Preventive Veterinary Medicine, São Paulo State University (Unesp), Rodovia de Acesso Paulo Donato Castellane s/n, Zona Rural, CEP 14884-012, Jaboticabal, SP, Brazil., da Rosa Prado EJ; Department of Preventive Veterinary Medicine, São Paulo State University (Unesp), Rodovia de Acesso Paulo Donato Castellane s/n, Zona Rural, CEP 14884-012, Jaboticabal, SP, Brazil., Montassier HJ; Department of Microbiology, São Paulo State University (Unesp), Rodovia de Acesso Paulo Donato Castellane s/n, Zona Rural, CEP 14884-012, Jaboticabal, SP, Brazil., Pilarski F; Laboratory of Aquatic Organisms Pathology (Lapoa), Aquaculture Center from UNESP (CAUNESP), Rodovia de Acesso Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil., Antonio de Andrade Belo M; Department of Preventive Veterinary Medicine, São Paulo State University (Unesp), Rodovia de Acesso Paulo Donato Castellane s/n, Zona Rural, CEP 14884-012, Jaboticabal, SP, Brazil; Laboratory of Animal Pharmacology and Toxicology, Brazil University, Av. Hilário da Silva Passos, 950, CEP.13690-000, Descalvado, SP, Brazil. Electronic address: maabelo@hotmail.com. |
| المصدر: | Fish & shellfish immunology [Fish Shellfish Immunol] 2020 Jun; Vol. 101, pp. 186-191. Date of Electronic Publication: 2020 Apr 01. |
| نوع المنشور: | Journal Article; Randomized Controlled Trial, Veterinary |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Academic Press, c1991- |
| مواضيع طبية MeSH: | Adaptive Immunity* , Characiformes* , Immunity, Innate*, Aeromonas hydrophila/*immunology , Bacterial Vaccines/*administration & dosage , Fish Diseases/*prevention & control , Gram-Negative Bacterial Infections/*veterinary , Vaccination/*veterinary, Animals ; Fish Diseases/immunology ; Fish Diseases/mortality ; Gram-Negative Bacterial Infections/immunology ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/prevention & control ; Immersion ; Injections, Intraperitoneal/veterinary ; Vaccines, Inactivated/administration & dosage |
| مستخلص: | Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses and protection against A. hydrophila in pacu, Piaractus mesopotamicus, vaccinated through intraperitoneal and immersion route with inactivated virulent strain. Fish were randomly distributed in three vaccinated groups: intraperitoneal (i.p.) route; immersion; and immersion + booster; and control group (unvaccinated). All vaccination protocols used the concentration of 1.7 × 10 8 CFU mL -1 of inactivated A. hydrophila., and an oil adjuvant was used for vaccine prepararion for i.p. route vaccination. Blood and skin mucus from 9 fishes per treatment were collected at 14, 28, 42 and 84 days post-vaccination (DPV) for determination of lysozyme concentration in skin mucus, as well as antibodies anti-A. hydrophila in blood serum and skin mucus. Fish were challenged at 84 DPV with homologous and virulent strain of A. hydrophila for evaluation of resistance against bacterial infection. The results demonstrated that vaccination with inactivated A. hydrophila suspension by i.p. or immersion resulted in significant increase of skin mucus lysozyme and specific antibody levels in serum and skin mucus, at 28 and 42 DPV, and this increase in innate and adaptive immunity remained significant in pacu vaccinated through i.p. route up to 84 DPV. Although no significant differences were observed in the survival study, pacu vaccinated through i.p. route presented 31,33% of relative percentage survival (RPS) in LD (Copyright © 2020 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aeromonadaceae; Antibodies; Characidae; Innate and adaptative immune; Lysozyme; Vaccination by immersion and i.p. route |
| المشرفين على المادة: | 0 (Bacterial Vaccines) 0 (Vaccines, Inactivated) |
| تواريخ الأحداث: | Date Created: 20200405 Date Completed: 20210112 Latest Revision: 20210112 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.fsi.2020.03.059 |
| PMID: | 32247044 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9947 |
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| DOI: | 10.1016/j.fsi.2020.03.059 |