دورية أكاديمية

Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis.

التفاصيل البيبلوغرافية
العنوان: Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis.
المؤلفون: Paschotto DR; Laboratory of Dentistry and Applied Materials (LDAM), Research and Development Institute (IP & D), Universidade do Vale do Paraíba, São José dos Campos, São Paulo, Brazil., Pupin B; Laboratory of Molecular Biology, Research and Development Institute (IP & D), Universidade do Vale do Paraiba, São José dos Campos, São Paulo, Brazil., Bhattacharjee TT; Sir John Walsh Research Institute, 310 Great King Street, Dunedin, 9016, New Zealand., Soares LES; Laboratory of Dentistry and Applied Materials (LDAM), Research and Development Institute (IP & D), Universidade do Vale do Paraíba, São José dos Campos, São Paulo, Brazil. lesoares@univap.br.
المصدر: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2020 Sep 10; Vol. 36 (9), pp. 1059-1064. Date of Electronic Publication: 2020 Apr 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Switzerland NLM ID: 8511078 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1348-2246 (Electronic) Linking ISSN: 09106340 NLM ISO Abbreviation: Anal Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2022- : [Cham] : Springer
Original Publication: Tokyo : The Society, [1985-
مواضيع طبية MeSH: Spectroscopy, Fourier Transform Infrared*, Saliva/*chemistry , Specimen Handling/*methods, Humans
مستخلص: Saliva has garnered a lot of interest as a non-invasive, easy to collect, and biochemical rich sample for attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) based disease diagnosis. Although a large number of studies have explored its potential, the preparation methods used differ greatly. For large scale clinical studies to aid translation into clinics, the collection/processing methodology needs to be standardized. Therefore, in this study, we explored different saliva collection (spitting, method A/cotton soaking, method B) and processing protocols (unprepared, TS; supernatant from the centrifugation, CS; and drying, C) to find which gives the best ATR-FTIR signals. Analysis showed highest proteins, carbohydrates, amino acids, and nucleic acid + proteins/lipids in BTS, BCS, ACS, and BC, respectively. Notably, only BC shows a 1377 cm -1 nucleic acid band that is also uniquely identified in multivariate analysis. We conclude that the collection-processing protocol should be based on a biochemical component that best gives a differential diagnosis.
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فهرسة مساهمة: Keywords: ATR-FTIR; best protocol; disease diagnosis; preparation methods; saliva; vibrational spectroscopy
تواريخ الأحداث: Date Created: 20200407 Date Completed: 20210625 Latest Revision: 20230808
رمز التحديث: 20240628
DOI: 10.2116/analsci.20P029
PMID: 32249246
قاعدة البيانات: MEDLINE
الوصف
تدمد:1348-2246
DOI:10.2116/analsci.20P029