دورية أكاديمية
أعرض في EDS

Knocking Down TcNTPDase-1 Gene Reduces in vitro Infectivity of Trypanosoma cruzi .

التفاصيل البيبلوغرافية
العنوان: Knocking Down TcNTPDase-1 Gene Reduces in vitro Infectivity of Trypanosoma cruzi .
المؤلفون: Silva-Gomes NL; Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Rampazzo RCP; Laboratory of Functional Genomics, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Moreira CMDN; Laboratory of Molecular Biology of Trypanosomatids, Carlos Chagas Institute, Curitiba, Brazil., Porcino GN; Laboratory of Structure and Function of Proteins, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Brazil., Dos Santos CMB; Laboratory of Functional Genomics, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Krieger MA; Laboratory of Functional Genomics, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Vasconcelos EG; Laboratory of Structure and Function of Proteins, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Brazil., Fragoso SP; Laboratory of Molecular Biology of Trypanosomatids, Carlos Chagas Institute, Curitiba, Brazil., Moreira OC; Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
المصدر: Frontiers in microbiology [Front Microbiol] 2020 Mar 18; Vol. 11, pp. 434. Date of Electronic Publication: 2020 Mar 18 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Ecto-Nucleoside Triphosphate Diphosphohydrolases are enzymes that hydrolyze tri- and/or diphosphate nucleosides. Evidences pointed out to their participation in Trypanosoma cruzi virulence, infectivity, and purine acquisition. In this study, recombinant T. cruzi knocking out or overexpressing the TcNTPDase-1 gene were built, and the role of TcNTPDase-1 in the in vitro interaction with VERO cells was investigated. Results show that epimastigote forms of hemi-knockout parasites showed about 50% lower level of TcNTPDase-1 gene expression when compared to the wild type, while the T. cruzi overexpressing this gene reach 20 times higher gene expression. In trypomastigote forms, the same decreasing in TcNTPDase-1 gene expression was observed to the hemi-knockout parasites. The in vitro infection assays showed a reduction to 51.6 and 59.9% at the adhesion and to 25.2 and 26.4% at the endocytic indexes to the parasites knockout to one or other allele (Hygro and Neo hemi-knockouts), respectively. In contrast, the infection assays with T. cruzi overexpressing TcNTPDase-1 from the WT or Neo hemi-knockout parasites showed an opposite result, with the increasing to 287.7 and 271.1% at the adhesion and to 220.4 and 186.7% at the endocytic indexes, respectively. The parasitic load estimated in infected VERO cells by quantitative real time PCR corroborated these findings. Taken together, the partial silencing and overexpression of the TcNTPDase-1 gene generated viable parasites with low and high infectivity rates, respectively, corroborating that the enzyme encoded for this gene plays an important role to the T. cruzi infectivity.
(Copyright © 2020 Silva-Gomes, Rampazzo, Moreira, Porcino, Santos, Krieger, Vasconcelos, Fragoso and Moreira.)
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فهرسة مساهمة: Keywords: TcNTPDase-1; Trypanosoma cruzi; infectivity; knockout; virulence
تواريخ الأحداث: Date Created: 20200408 Latest Revision: 20200928
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7094052
DOI: 10.3389/fmicb.2020.00434
PMID: 32256481
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2020.00434