دورية أكاديمية
أعرض في EDS

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation.

التفاصيل البيبلوغرافية
العنوان: MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation.
المؤلفون: Greither T; Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany., Wenzel C; Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany., Jansen J; Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany., Kraus M; Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany., Wabitsch M; Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Ulm, Germany., Behre HM; Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany.
المصدر: Adipocyte [Adipocyte] 2020 Dec; Vol. 9 (1), pp. 197-205.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101567863 Publication Model: Print Cited Medium: Internet ISSN: 2162-397X (Electronic) Linking ISSN: 21623945 NLM ISO Abbreviation: Adipocyte Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin, TX : Landes Bioscience
مواضيع طبية MeSH: Adipogenesis*, Adipocytes/*metabolism , Androgens/*metabolism , MicroRNAs/*metabolism, Cells, Cultured ; Humans ; MicroRNAs/genetics
مستخلص: Objectives : Adipogenesis is the differentiation process generating mature adipocytes from undifferentiated mesenchymal stem cells. The differentiation can be inhibited by androgens, although knowledge about intracellular effectors of this inhibition is scarce. Recently, androgen-regulated microRNAs were detected as interesting candidates in this context. In this study, we analyse the role of miR-130a and miR-301 in the adipogenesis of human SGBS preadipocytes and whether they are prone to androgen regulation. Materials and Methods : microRNA expression during adipogenic differentiation with or without androgen stimulation was measured by qPCR. Putative target genes of miR-130a and miR-301 were identified by target database search and validated in luciferase reporter assays. Results : miR-130a and miR-301 are both significantly downregulated on day 3 and day 5 of adipogenic differentiation in comparison to day 0. Under androgen stimulation, a significant upregulation of miR-130a was detected after 7 days of adipogenesis lasting to day 14, while miR-301 did not change significantly until day 14. Luciferase reporter assays revealed the androgen receptor (AR), adiponectin (ADIPOQ) and tumour necrosis factor alpha (TNFα) as miR-130a target genes. Conclusions : miR-130a is an androgen-regulated microRNA that is downregulated during the early phase of adipogenesis and exerts its functions by regulating AR and ADIPOQ translation. These data may help to identify new signalling pathways associated with the androgen-mediated inhibition of adipogenesis.
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فهرسة مساهمة: Keywords: Adipogenesis; androgens; miR-130a; microRNA
المشرفين على المادة: 0 (Androgens)
0 (MIRN130 microRNA, human)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20200411 Date Completed: 20210223 Latest Revision: 20210422
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7153545
DOI: 10.1080/21623945.2020.1750256
PMID: 32272867
قاعدة البيانات: MEDLINE
الوصف
تدمد:2162-397X
DOI:10.1080/21623945.2020.1750256