دورية أكاديمية
أعرض في EDS

First report of VIM metallo-β-lactamase production in Escherichia coli and Klebsiella pneumoniae clinical isolates from Gaza Strip, Palestine.

التفاصيل البيبلوغرافية
العنوان: First report of VIM metallo-β-lactamase production in Escherichia coli and Klebsiella pneumoniae clinical isolates from Gaza Strip, Palestine.
المؤلفون: Tayh G; PhD, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie., Nagarjuna D; PhD, Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi-110007, India., Sallem RB; PhD, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie., Verma V; PhD, Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi-110007, India., Chairat S; MSc, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie., Boudabous A; PhD, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie., Yadav M; PhD, Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi-110007, India., Slama KB; PhD, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie.
المصدر: Germs [Germs] 2020 Mar 02; Vol. 10 (1), pp. 18-26. Date of Electronic Publication: 2020 Mar 02 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: European Academy of HIV/AIDS and Infectious Diseases Country of Publication: Romania NLM ID: 101596099 Publication Model: eCollection Cited Medium: Print ISSN: 2248-2997 (Print) Linking ISSN: 22482997 NLM ISO Abbreviation: Germs Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Bucureşti : European Academy of HIV/AIDS and Infectious Diseases
مستخلص: Introduction: Even though the increasing incidence of VIM-producing E. coli and K. pneumoniae has been reported worldwide, studies are still lacking in Palestine. The aim of this study was to screen carbapenem-resistant E. coli and K. pneumoniae bacteria in the Gaza Strip, Palestine and further to characterize carbapenemase-producing isolates.
Methods: A total of 69 E. coli and 27 K. pneumoniae isolates were obtained from three Gaza hospitals and recovered from urine, wound swabs, blood and ear discharge. The screening for metallo-β-lactamases (MBLs) was performed by using the imipenem-EDTA disc synergy test. The detection of β-lactamases genes, detection of non-β-lactam genes and the characterization of integrons were performed by PCR and sequencing. The clonal relationship among the isolates was determined by pulsed-field gel electrophoresis (PFGE).
Results: Our study showed that 4 E. coli (5.8%) and 5 K. pneumoniae (18.5%) were positive by the imipenem-EDTA disc synergy test. Bla VIM-4 was detected in six isolates and bla VIM-28 was identified in three isolates. The β-lactamases genes in the VIM-producing K. pneumoniae isolates were bla CTX-M-15 (n=3), bla CTX-M-14 (n=1), bla SHV-1 (n=3), bla SHV-12 (n=1), bla TEM-1 (n=1) and bla OXA-1 (n=1). Aac(6') -Ib-cr gene was confirmed in four E. coli and in two K. pneumoniae isolates. QnrS1 was identified in two K. pneumoniae isolates. The class 1 integron was identified with the different gene cassette; dfrA17-aadA5, dfrA5, dfrA12-orf-aadA2 and dfrA17-aadA5 were identified.
Conclusions: Our study indicated for the first time the emergence of multidrug-resistant VIM-containing K. pneumoniae and E. coli isolates of clinical origin in Gaza Strip hospitals.
Competing Interests: Conflicts of interest: All authors – none to declare.
(GERMS.)
References: Clin Microbiol Rev. 2005 Apr;18(2):306-25. (PMID: 15831827)
Diagn Microbiol Infect Dis. 2004 Mar;48(3):167-72. (PMID: 15023424)
New Microbes New Infect. 2019 Feb 22;29:100521. (PMID: 30976429)
Clin Microbiol Infect. 2002 Jun;8(6):321-31. (PMID: 12084099)
Antimicrob Agents Chemother. 2006 Dec;50(12):4198-201. (PMID: 17015633)
J Med Microbiol. 2013 Aug;62(Pt 8):1239-1244. (PMID: 23639985)
Antimicrob Agents Chemother. 2003 Jan;47(1):395-7. (PMID: 12499222)
Burns. 2013 Dec;39(8):1612-8. (PMID: 23664775)
Open Microbiol J. 2018 Jan 31;12:1-7. (PMID: 29456773)
J Glob Antimicrob Resist. 2014 Jun;2(2):93-97. (PMID: 27873597)
J Glob Antimicrob Resist. 2018 Mar;12:107-112. (PMID: 28986323)
J Antimicrob Chemother. 2010 Oct;65(10):2070-5. (PMID: 20656680)
Can J Infect Dis Med Microbiol. 2016;2016:4379686. (PMID: 27597874)
Rev Esp Quimioter. 2008 Jun;21(2):123-6. (PMID: 18509771)
J Antimicrob Chemother. 2007 Nov;60(5):1137-41. (PMID: 17855726)
Antimicrob Agents Chemother. 2015 Jul;59(7):4305-7. (PMID: 25896692)
Int J Infect Dis. 2013 Dec;17(12):e1130-3. (PMID: 24021566)
Jpn J Infect Dis. 2014;67(5):382-4. (PMID: 25241690)
J Infect Dev Ctries. 2018 Jul 31;12(7):520-525. (PMID: 31954000)
J Mol Biol. 2019 Aug 23;431(18):3472-3500. (PMID: 30959050)
فهرسة مساهمة: Keywords: CTX-M ESBL; Palestine; VIM metallo-β-lactamase; antimicrobial resistance; carbapenem; integron
تواريخ الأحداث: Date Created: 20200411 Latest Revision: 20220413
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7117887
DOI: 10.18683/germs.2020.1181
PMID: 32274356
قاعدة البيانات: MEDLINE
الوصف
تدمد:2248-2997
DOI:10.18683/germs.2020.1181